7pcw
From Proteopedia
(Difference between revisions)
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==X-RAY STRUCTURE OF THE HALOALKANE DEHALOGENASE HALOTAG7-M175W LABELED WITH A CHLOROALKANE-TETRAMETHYLRHODAMINE FLUOROPHORE SUBSTRATE== | ==X-RAY STRUCTURE OF THE HALOALKANE DEHALOGENASE HALOTAG7-M175W LABELED WITH A CHLOROALKANE-TETRAMETHYLRHODAMINE FLUOROPHORE SUBSTRATE== | ||
| - | <StructureSection load='7pcw' size='340' side='right'caption='[[7pcw]]' scene=''> | + | <StructureSection load='7pcw' size='340' side='right'caption='[[7pcw]], [[Resolution|resolution]] 2.30Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PCW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PCW FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7pcw]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PCW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PCW FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pcw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pcw OCA], [https://pdbe.org/7pcw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pcw RCSB], [https://www.ebi.ac.uk/pdbsum/7pcw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pcw ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=OEH:[9-[2-carboxy-5-[2-[2-(6-chloranylhexoxy)ethoxy]ethylcarbamoyl]phenyl]-6-(dimethylamino)xanthen-3-ylidene]-dimethyl-azanium'>OEH</scene></td></tr> |
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[6zvu|6zvu]], [[6zvv|6zvv]], [[6zvw|6zvw]], [[6zvx|6zvx]], [[6zvy|6zvy]], [[7pcx|7pcx]]</div></td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Haloalkane_dehalogenase Haloalkane dehalogenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.8.1.5 3.8.1.5] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pcw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pcw OCA], [https://pdbe.org/7pcw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pcw RCSB], [https://www.ebi.ac.uk/pdbsum/7pcw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pcw ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Self-labeling protein tags such as HaloTag are powerful tools that can label fusion proteins with synthetic fluorophores for use in fluorescence microscopy. Here we introduce HaloTag variants with either increased or decreased brightness and fluorescence lifetime compared with HaloTag7 when labeled with rhodamines. Combining these HaloTag variants enabled live-cell fluorescence lifetime multiplexing of three cellular targets in one spectral channel using a single fluorophore and the generation of a fluorescence lifetime-based biosensor. Additionally, the brightest HaloTag variant showed up to 40% higher brightness in live-cell imaging applications. | ||
| + | |||
| + | Engineered HaloTag variants for fluorescence lifetime multiplexing.,Frei MS, Tarnawski M, Roberti MJ, Koch B, Hiblot J, Johnsson K Nat Methods. 2022 Jan;19(1):65-70. doi: 10.1038/s41592-021-01341-x. Epub 2021 Dec, 16. PMID:34916672<ref>PMID:34916672</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7pcw" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Haloalkane dehalogenase]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Frei M]] | + | [[Category: Frei, M]] |
| - | [[Category: Hiblot J]] | + | [[Category: Hiblot, J]] |
| - | [[Category: Johnsson K]] | + | [[Category: Johnsson, K]] |
| - | [[Category: Tarnawski M]] | + | [[Category: Tarnawski, M]] |
| + | [[Category: Fluorophore]] | ||
| + | [[Category: Halo]] | ||
| + | [[Category: Halotag7]] | ||
| + | [[Category: Hydrolase]] | ||
| + | [[Category: Self-labeling protein]] | ||
| + | [[Category: Tag]] | ||
| + | [[Category: Tetramethylrhodamine]] | ||
Revision as of 11:06, 16 February 2022
X-RAY STRUCTURE OF THE HALOALKANE DEHALOGENASE HALOTAG7-M175W LABELED WITH A CHLOROALKANE-TETRAMETHYLRHODAMINE FLUOROPHORE SUBSTRATE
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