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| - | [[Image:1fyb.jpg|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1fyb| PDB=1fyb | SCENE= }} | | {{STRUCTURE_1fyb| PDB=1fyb | SCENE= }} |
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| - | '''SOLUTION STRUCTURE OF C1-T1, A TWO-DOMAIN PROTEINASE INHIBITOR DERIVED FROM THE CIRCULAR PRECURSOR PROTEIN NA-PROPI FROM NICOTIANA ALATA'''
| + | ===SOLUTION STRUCTURE OF C1-T1, A TWO-DOMAIN PROTEINASE INHIBITOR DERIVED FROM THE CIRCULAR PRECURSOR PROTEIN NA-PROPI FROM NICOTIANA ALATA=== |
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| - | ==Overview==
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| - | A two-domain portion of the proteinase inhibitor precursor from Nicotiana alata (NaProPI) has been expressed and its structure determined by NMR spectroscopy. NaProPI contains six almost identical 53 amino acid repeats that fold into six highly similar domains; however, the sequence repeats do not coincide with the structural domains. Five of the structural domains comprise the C-terminal portion of one repeat and the N-terminal portion of the next. The sixth domain contains the C-terminal portion of the sixth repeat and the N-terminal portion of the first repeat. Disulphide bonds link these C and N-terminal fragments to generate the clasped-bracelet fold of NaProPI. The three-dimensional structure of NaProPI is not known, but it is conceivable that adjacent domains in NaProPI interact to generate the circular "bracelet" with the N and C termini in close enough proximity to facilitate formation of the disulphide bonds that form the "clasp". The expressed protein, examined in the current study, comprises residues 25-135 of NaProPI and encompasses the first two contiguous structural domains, namely the chymotrypsin inhibitor C1 and the trypsin inhibitor T1, joined by a five-residue linker, and is referred to as C1-T1. The tertiary structure of each domain in C1-T1 is identical to that found in the isolated inhibitors. However, no nuclear Overhauser effect contacts are observed between the two domains and the five-residue linker adopts an extended conformation. The absence of interactions between the domains indicates that adjacent domains do not specifically interact to drive the circularisation of NaProPI. These results are in agreement with recent data which describe similar PI precursors from other members of the Solanaceae having two, three, or four repeats. The lack of strong interdomain association is likely to be important for the function of individual inhibitors by ensuring that there is no masking of reactive sites upon release from the precursor.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_11178894}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 11178894 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_11178894}} |
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| | ==About this Structure== | | ==About this Structure== |
| - | 1FYB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Nicotiana_alata Nicotiana alata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FYB OCA]. | + | 1FYB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Nicotiana_alata Nicotiana alata]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FYB OCA]. |
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| | ==Reference== | | ==Reference== |
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| | [[Category: Schirra, H J.]] | | [[Category: Schirra, H J.]] |
| | [[Category: Two-domain protein]] | | [[Category: Two-domain protein]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:54:14 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 04:07:32 2008'' |
Revision as of 01:07, 1 July 2008
Template:STRUCTURE 1fyb
SOLUTION STRUCTURE OF C1-T1, A TWO-DOMAIN PROTEINASE INHIBITOR DERIVED FROM THE CIRCULAR PRECURSOR PROTEIN NA-PROPI FROM NICOTIANA ALATA
Template:ABSTRACT PUBMED 11178894
About this Structure
1FYB is a Single protein structure of sequence from Nicotiana alata. Full experimental information is available from OCA.
Reference
The solution structure of C1-T1, a two-domain proteinase inhibitor derived from a circular precursor protein from Nicotiana alata., Schirra HJ, Scanlon MJ, Lee MC, Anderson MA, Craik DJ, J Mol Biol. 2001 Feb 9;306(1):69-79. PMID:11178894
Page seeded by OCA on Tue Jul 1 04:07:32 2008
