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| - | [[Image:1fzs.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1fzs| PDB=1fzs | SCENE= }} | | {{STRUCTURE_1fzs| PDB=1fzs | SCENE= }} |
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| - | '''DNA WITH PYRENE PAIRED AT ABASIC SITE'''
| + | ===DNA WITH PYRENE PAIRED AT ABASIC SITE=== |
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| - | ==Overview==
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| - | DNA polymerases specifically insert the hydrophobic pyrene deoxynucleotide (P) opposite tetrahydrofuran (F), an stable abasic site analog, and DNA duplexes containing this non-hydrogen-bonded pair possess a high degree of thermodynamic stability. These observations support the hypothesis that steric complementarity and stacking interactions may be sufficient for maintaining stability of DNA structure and specificity of DNA replication, even in the absence of hydrogen bonds across the base pair. Here we report the NMR characterization and structure determination of two DNA molecules containing pyrene residues. The first is a 13mer duplex with a pyrene.tetrahydrofuran pair (P.F pair) at the ninth position and the second mimics a replication intermediate right after incorporation of a pyrene nucleoside opposite an abasic site. Our data indicate that both molecules adopt right-handed helical conformations with Watson- Crick alignments for all canonical base pairs. The pyrene ring stays inside the helix close to its baseless partner in both molecules. The single-stranded region of the replication intermediate folds back over the opposing strand, sheltering the hydrophobic pyrene moiety from water exposure. The results support the idea that the stability and replication of a P.F pair is due to its ability to mimic Watson-Crick structure.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_12490724}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 12490724 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_12490724}} |
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| | ==About this Structure== | | ==About this Structure== |
| - | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FZS OCA]. | + | Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FZS OCA]. |
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| | ==Reference== | | ==Reference== |
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| | [[Category: Non-polar dna pair]] | | [[Category: Non-polar dna pair]] |
| | [[Category: Pyrene]] | | [[Category: Pyrene]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:56:58 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 04:11:08 2008'' |
Revision as of 01:11, 1 July 2008
Template:STRUCTURE 1fzs
DNA WITH PYRENE PAIRED AT ABASIC SITE
Template:ABSTRACT PUBMED 12490724
About this Structure
Full experimental information is available from OCA.
Reference
Integrity of duplex structures without hydrogen bonding: DNA with pyrene paired at abasic sites., Smirnov S, Matray TJ, Kool ET, de los Santos C, Nucleic Acids Res. 2002 Dec 15;30(24):5561-9. PMID:12490724
Page seeded by OCA on Tue Jul 1 04:11:08 2008
