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3a54

From Proteopedia

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Crystal structure of the A47Q1 mutant of pro-protein-glutaminase

Structural highlights

3a54 is a 2 chain structure with sequence from Chryseobacterium proteolyticum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.502Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q9AQQ8_9FLAO

Publication Abstract from PubMed

Protein glutaminase, which converts a protein glutamine residue to a glutamate residue, is expected to be useful as a new food-processing enzyme. The crystal structures of the mature and pro forms of the enzyme were refined at 1.15 and 1.73 A resolution, respectively. The overall structure of the mature enzyme has a weak homology to the core domain of human transglutaminase-2. The catalytic triad (Cys-His-Asp) common to transglutaminases and cysteine proteases is located in the bottom of the active site pocket. The structure of the recombinant pro form shows that a short loop between S2 and S3 in the proregion covers and interacts with the active site of the mature region, mimicking the protein substrate of the enzyme. Ala-47 is located just above the pocket of the active site. Two mutant structures (A47Q-1 and A47Q-2) refined at 1.5 A resolution were found to correspond to the enzyme-substrate complex and an S-acyl intermediate. Based on these structures, the catalytic mechanism of protein glutaminase is proposed.

Crystal structures of protein glutaminase and its pro forms converted into enzyme-substrate complex.,Hashizume R, Maki Y, Mizutani K, Takahashi N, Matsubara H, Sugita A, Sato K, Yamaguchi S, Mikami B J Biol Chem. 2011 Nov 4;286(44):38691-702. Epub 2011 Sep 16. PMID:21926168^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hashizume R, Maki Y, Mizutani K, Takahashi N, Matsubara H, Sugita A, Sato K, Yamaguchi S, Mikami B. Crystal structures of protein glutaminase and its pro forms converted into enzyme-substrate complex. J Biol Chem. 2011 Nov 4;286(44):38691-702. Epub 2011 Sep 16. PMID:21926168 doi:10.1074/jbc.M111.255133

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3a54"

Categories: Chryseobacterium proteolyticum | Large Structures | Hashizume R | Mikami B | Yamaguchi S

@@ Line 3: / Line 3: @@
 <StructureSection load='3a54' size='340' side='right'caption='[[3a54]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3a54]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"chryseobacterium_proteolyticum"_yamaguchi_and_yokoe_2000 "chryseobacterium proteolyticum" yamaguchi and yokoe 2000]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A54 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3A54 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3a54]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Chryseobacterium_proteolyticum Chryseobacterium proteolyticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A54 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3A54 FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2zk9|2zk9]], [[3a55|3a55]], [[3a56|3a56]]</div></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.502&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">prgA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=118127 "Chryseobacterium proteolyticum" Yamaguchi and Yokoe 2000])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3a54 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a54 OCA], [https://pdbe.org/3a54 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3a54 RCSB], [https://www.ebi.ac.uk/pdbsum/3a54 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3a54 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q9AQQ8_9FLAO Q9AQQ8_9FLAO]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 24: / Line 25: @@
 __TOC__
 </StructureSection>
-[[Category: Chryseobacterium proteolyticum yamaguchi and yokoe 2000]]
+[[Category: Chryseobacterium proteolyticum]]
 [[Category: Large Structures]]
-[[Category: Hashizume, R]]
+[[Category: Hashizume R]]
-[[Category: Mikami, B]]
+[[Category: Mikami B]]
-[[Category: Yamaguchi, S]]
+[[Category: Yamaguchi S]]
-[[Category: Hydrolase]]
-[[Category: Mutant structure like a substrate-enzyme complex]]

3a54

From Proteopedia

Current revision

Crystal structure of the A47Q1 mutant of pro-protein-glutaminase

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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