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| | {{STRUCTURE_1g15| PDB=1g15 | SCENE= }} | | {{STRUCTURE_1g15| PDB=1g15 | SCENE= }} |
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| - | '''CO-CRYSTAL OF E. COLI RNASE HI WITH TWO MN2+ IONS BOUND IN THE THE ACTIVE SITE'''
| + | ===CO-CRYSTAL OF E. COLI RNASE HI WITH TWO MN2+ IONS BOUND IN THE THE ACTIVE SITE=== |
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| - | ==Overview==
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| - | Ribonuclease H (RNase H) selectively degrades the RNA strand of RNA.DNA hybrids in a divalent cation-dependent manner. Previous structural studies revealed a single Mg(2+) ion-binding site in Escherichia coli RNase HI. In the crystal structure of the related RNase H domain of human immunodeficiency virus reverse transcriptase, however, two Mn(2+) ions were observed suggesting a different mode of metal binding. E. coli RNase HI shows catalytic activity in the presence of Mg(2+) or Mn(2+) ions, but these two metals show strikingly different optimal concentrations. Mg(2+) ions are required in millimolar concentrations, but Mn(2+) ions are only required in micromolar quantities. Based upon the metal dependence of E. coli RNase HI activity, we proposed an activation/attenuation model in which one metal is required for catalysis, and binding of a second metal is inhibitory. We have now solved the co-crystal structure of E. coli RNase HI with Mn(2+) ions at 1.9-A resolution. Two octahedrally coordinated Mn(2+) ions are seen to bind to the enzyme-active site. Residues Asp-10, Glu-48, and Asp-70 make direct (inner sphere) coordination contacts to the first (activating) metal, whereas residues Asp-10 and Asp-134 make direct contacts to the second (attenuating) metal. This structure is consistent with biochemical evidence suggesting that two metal ions may bind RNase H but liganding a second ion inhibits RNase H activity.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_11083878}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 11083878 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_11083878}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Ribonuclease h]] | | [[Category: Ribonuclease h]] |
| | [[Category: Rnase h]] | | [[Category: Rnase h]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:00:12 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 04:15:03 2008'' |
Revision as of 01:15, 1 July 2008
Template:STRUCTURE 1g15
CO-CRYSTAL OF E. COLI RNASE HI WITH TWO MN2+ IONS BOUND IN THE THE ACTIVE SITE
Template:ABSTRACT PUBMED 11083878
About this Structure
1G15 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Co-crystal of Escherichia coli RNase HI with Mn2+ ions reveals two divalent metals bound in the active site., Goedken ER, Marqusee S, J Biol Chem. 2001 Mar 9;276(10):7266-71. Epub 2000 Nov 16. PMID:11083878
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