2y0r

From Proteopedia

(Difference between revisions)

Current revision

Structural basis for the allosteric interference of myosin function by mutants G680A and G680V of Dictyostelium myosin-2

Structural highlights

2y0r is a 1 chain structure with sequence from Dictyostelium discoideum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.85Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

MYS2_DICDI Myosin is a protein that binds to actin and has ATPase activity that is activated by actin.

Publication Abstract from PubMed

The cold-sensitive single-residue mutation of glycine 680 in the reactive thiol region of Dictyostelium discoideum myosin-2 or the corresponding conserved glycine in other myosin isoforms has been reported to interfere with motor function. Here we present the x-ray structures of myosin motor domain mutants G680A in the absence and presence of nucleotide as well as the apo structure of mutant G680V. Our results show that the Gly-680 mutations lead to uncoupling of the reactive thiol region from the surrounding structural elements. Structural and functional data indicate that the mutations induce the preferential population of a state that resembles the ADP-bound state. Moreover, the Gly-680 mutants display greatly reduced dynamic properties, which appear to be related to the recovery of myosin motor function at elevated temperatures.

Structural Basis for the Allosteric Interference of Myosin Function by Reactive Thiol Region Mutations G680A and G680V.,Preller M, Bauer S, Adamek N, Fujita-Becker S, Fedorov R, Geeves MA, Manstein DJ J Biol Chem. 2011 Oct 7;286(40):35051-60. Epub 2011 Aug 13. PMID:21841195^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Preller M, Bauer S, Adamek N, Fujita-Becker S, Fedorov R, Geeves MA, Manstein DJ. Structural Basis for the Allosteric Interference of Myosin Function by Reactive Thiol Region Mutations G680A and G680V. J Biol Chem. 2011 Oct 7;286(40):35051-60. Epub 2011 Aug 13. PMID:21841195 doi:10.1074/jbc.M111.265298

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2y0r"

@@ Line 3: / Line 3: @@
 <StructureSection load='2y0r' size='340' side='right'caption='[[2y0r]], [[Resolution|resolution]] 2.85&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2y0r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_11735 Atcc 11735]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y0R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Y0R FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2y0r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y0R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Y0R FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1mne|1mne]], [[1d1c|1d1c]], [[1jx2|1jx2]], [[1q5g|1q5g]], [[1w9l|1w9l]], [[1vom|1vom]], [[1d0y|1d0y]], [[1mmn|1mmn]], [[2aka|2aka]], [[2jhr|2jhr]], [[2jj9|2jj9]], [[2xel|2xel]], [[1lvk|1lvk]], [[1w9j|1w9j]], [[1jwy|1jwy]], [[2x9h|2x9h]], [[1fmv|1fmv]], [[1yv3|1yv3]], [[1d0x|1d0x]], [[1fmw|1fmw]], [[1d1b|1d1b]], [[1g8x|1g8x]], [[1mma|1mma]], [[1d1a|1d1a]], [[1mmg|1mmg]], [[2xo8|2xo8]], [[1w9i|1w9i]], [[1d0z|1d0z]], [[1mnd|1mnd]], [[1w9k|1w9k]], [[1mmd|1mmd]], [[2y8i|2y8i]], [[2y9e|2y9e]]</div></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.85&#8491;</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2y0r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y0r OCA], [https://pdbe.org/2y0r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2y0r RCSB], [https://www.ebi.ac.uk/pdbsum/2y0r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2y0r ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/MYS2_DICDI MYS2_DICDI]] Myosin is a protein that binds to actin and has ATPase activity that is activated by actin.
+[https://www.uniprot.org/uniprot/MYS2_DICDI MYS2_DICDI] Myosin is a protein that binds to actin and has ATPase activity that is activated by actin.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 25: / Line 25: @@
 __TOC__
 </StructureSection>
-[[Category: Atcc 11735]]
+[[Category: Dictyostelium discoideum]]
 [[Category: Large Structures]]
-[[Category: Adamek, N]]
+[[Category: Adamek N]]
-[[Category: Bauer, S]]
+[[Category: Bauer S]]
-[[Category: Fedorov, R]]
+[[Category: Fedorov R]]
-[[Category: Fujita-Becker, S]]
+[[Category: Fujita-Becker S]]
-[[Category: Geeves, M A]]
+[[Category: Geeves MA]]
-[[Category: Manstein, D J]]
+[[Category: Manstein DJ]]
-[[Category: Preller, M]]
+[[Category: Preller M]]
-[[Category: Motor protein]]

2y0r

From Proteopedia

Current revision

Structural basis for the allosteric interference of myosin function by mutants G680A and G680V of Dictyostelium myosin-2

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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