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1g1k

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{{STRUCTURE_1g1k| PDB=1g1k | SCENE= }}
{{STRUCTURE_1g1k| PDB=1g1k | SCENE= }}
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'''COHESIN MODULE FROM THE CELLULOSOME OF CLOSTRIDIUM CELLULOLYTICUM'''
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===COHESIN MODULE FROM THE CELLULOSOME OF CLOSTRIDIUM CELLULOLYTICUM===
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==Overview==
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In the assembly of the Clostridium cellulolyticum cellulosome, the multiple cohesin modules of the scaffolding protein CipC serve as receptors for cellulolytic enzymes which bear a dockerin module. The X-ray structure of a type I C. cellulolyticum cohesin module (Cc-cohesin) has been solved using molecular replacement, and refined at 2.0 A resolution. Despite a rather low sequence identity of 32 %, this module has a fold close to those of the two Clostridium thermocellum cohesin (Ct-cohesin) modules whose 3D structures have been determined previously. Cc-cohesin forms a dimer in the crystal, as do the two Ct-cohesins. We show here that the dimer exists in solution and that addition of dockerin-containing proteins dissociates the dimer. This suggests that the dimerization interface and the cohesin/dockerin interface may overlap. The nature of the overall surface and of the dimer interface of Cc-cohesin differ notably from those of the Ct-cohesin modules, being much less polar, and this may explain the species specificity observed in the cohesin/dockerin interaction of C. cellulolyticum and C. thermocellum. We have produced a topology model of a C. cellulolyticum dockerin and of a Cc-cohesin/dockerin complex using homology modeling and available biochemical data. Our model suggests that a special residue pair, already identified in dockerin sequences, is located at the center of the cohesin surface putatively interacting with the dockerin.
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{{ABSTRACT_PUBMED_11080455}}
==About this Structure==
==About this Structure==
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[[Category: Spinelli, S.]]
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[[Category: Beta -barrel]]
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Revision as of 01:16, 1 July 2008

Image:1g1k.png

Template:STRUCTURE 1g1k

COHESIN MODULE FROM THE CELLULOSOME OF CLOSTRIDIUM CELLULOLYTICUM

Template:ABSTRACT PUBMED 11080455

About this Structure

1G1K is a Single protein structure of sequence from Clostridium cellulolyticum. Full crystallographic information is available from OCA.

Reference

Crystal structure of a cohesin module from Clostridium cellulolyticum: implications for dockerin recognition., Spinelli S, Fierobe HP, Belaich A, Belaich JP, Henrissat B, Cambillau C, J Mol Biol. 2000 Nov 24;304(2):189-200. PMID:11080455

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