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1g26

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[[Image:1g26.gif|left|200px]]
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{{STRUCTURE_1g26| PDB=1g26 | SCENE= }}
{{STRUCTURE_1g26| PDB=1g26 | SCENE= }}
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'''THE SOLUTION STRUCTURE OF A WELL-FOLDED PEPTIDE BASED ON THE 31-RESIDUE AMINO-TERMINAL SUBDOMAIN OF HUMAN GRANULIN A'''
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===THE SOLUTION STRUCTURE OF A WELL-FOLDED PEPTIDE BASED ON THE 31-RESIDUE AMINO-TERMINAL SUBDOMAIN OF HUMAN GRANULIN A===
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==Overview==
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Four amino acid substitutions were introduced into a peptide corresponding to the amino-terminal subdomain (30-31 residues) of human granulin A (HGA) in order to assess the contributions of a hydrophobic framework and other interactions to structure stabilization of the stack of two beta-hairpins. The resulting hybrid peptide, HGA 1-31 (D1V, K3H, S9I, Q20P) with four free cysteines, spontaneously formed a uniquely disulfide-bonded isomer with an expected [1-3, 2-4] disulfide pairing pattern. This peptide was characterized in detail by use of NMR and shown to assume a highly stable structure in solution, in contrast to the amino-terminal 1-30 fragment of human granulin A. The prototype peptide, or HGA 1-30 (C17S, C27S), had lower resistance to chemical reduction and proteolysis, broad NH and H(alpha) proton resonances, lower proton resonance dispersion, and no slowly exchanging amide protons. Two other peptides, HGA 1-30 (C17S, Q20P, C27S) and HGA 1-31 (D1V, K3H, S9I, C17S, C27S), with either Pro20 stabilizing a potential reverse turn or with a hydrophobic cluster consisting of Val1, His3, and Ile9, had sharper and slightly better dispersed NH and H(alpha) proton resonances, but still no slowly exchanging amide protons. The solution structure of HGA 1-31 (D1V, K3H, S9I, Q20P) indicates that it adopts a well-folded conformation of a stack of two beta-hairpins, as found for the amino-terminal subdomain of the prototypic carp granulin-1 with representative beta-hairpin stacks. These results highlight the importance of both hydrophobic and turn-stabilizing interactions for the structural integrity of the hairpin stack scaffold. The conformational stability appears to be maintained by a combination of the well-formed second beta-hairpin and two hydrophobic clusters, one located at the interface between the two beta-hairpins and the other on "top" of the first beta-hairpin. The implications of these findings for the design of conformationally stable hairpin stacks are discussed.
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{{ABSTRACT_PUBMED_10715107}}
==About this Structure==
==About this Structure==
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1G26 is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G26 OCA].
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1G26 is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G26 OCA].
==Reference==
==Reference==
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[[Category: Beta-hairpin stack]]
[[Category: Beta-hairpin stack]]
[[Category: Granulin/epithelin protein repeat]]
[[Category: Granulin/epithelin protein repeat]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:02:34 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 04:17:50 2008''

Revision as of 01:17, 1 July 2008

Image:1g26.png

Template:STRUCTURE 1g26

THE SOLUTION STRUCTURE OF A WELL-FOLDED PEPTIDE BASED ON THE 31-RESIDUE AMINO-TERMINAL SUBDOMAIN OF HUMAN GRANULIN A

Template:ABSTRACT PUBMED 10715107

About this Structure

1G26 is a Single protein structure. Full experimental information is available from OCA.

Reference

Design and solution structure of a well-folded stack of two beta-hairpins based on the amino-terminal fragment of human granulin A., Tolkatchev D, Ng A, Vranken W, Ni F, Biochemistry. 2000 Mar 21;39(11):2878-86. PMID:10715107

Page seeded by OCA on Tue Jul 1 04:17:50 2008

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