2gx0

From Proteopedia

(Difference between revisions)

Revision as of 08:49, 25 October 2023

Crystal structural and functional analysis of GFP-like fluorescent protein

Structural highlights

2gx0 is a 4 chain structure with sequence from Echinophyllia sp. SC22. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.9Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q5TLG6_9CNID

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The photoswitching behavior of green fluorescent proteins (GFPs) or GFP-like proteins is increasingly recognized as a new technique for optical marking. Recently, Ando and his colleagues developed a new green fluorescent protein Dronpa, which possesses the unique photochromic property of being photoswitchable in a non-destructive manner. To better understand this mechanism, we determined the crystal structures of a new GFP Dronpa and its mutant C62S, at 1.9 Angstroms and 1.8 Angstroms, respectively. Determination of the structures demonstrates that a unique hydrogen-bonding network and the sulfur atom of the chromophore are critical to the photoswitching property of Dronpa. Reversible photoswitching was lost in cells expressing the Dronpa-C62S upon repetitive irradiation compared to the native protein. Structural and mutational analyses reveal the chemical basis for the functional properties of photoswitchable fluorescent proteins and provide the basis for subsequent coherent engineering of this subfamily of Dronpa homologs.

Structural characterization of the photoswitchable fluorescent protein Dronpa-C62S.,Nam KH, Kwon OY, Sugiyama K, Lee WH, Kim YK, Song HK, Kim EE, Park SY, Jeon H, Hwang KY Biochem Biophys Res Commun. 2007 Mar 23;354(4):962-7. Epub 2007 Jan 24. PMID:17276392^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Nam KH, Kwon OY, Sugiyama K, Lee WH, Kim YK, Song HK, Kim EE, Park SY, Jeon H, Hwang KY. Structural characterization of the photoswitchable fluorescent protein Dronpa-C62S. Biochem Biophys Res Commun. 2007 Mar 23;354(4):962-7. Epub 2007 Jan 24. PMID:17276392 doi:10.1016/j.bbrc.2007.01.086

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2gx0"

@@ Line 3: / Line 3: @@
 <StructureSection load='2gx0' size='340' side='right'caption='[[2gx0]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2gx0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Echinophyllia_sp._sc22 Echinophyllia sp. sc22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GX0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GX0 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2gx0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Echinophyllia_sp._SC22 Echinophyllia sp. SC22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GX0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GX0 FirstGlance]. <br>
-</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=GYC:[(4Z)-2-[(1R)-1-AMINO-2-MERCAPTOETHYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>GYC</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2gx2|2gx2]]</div></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GYC:[(4Z)-2-[(1R)-1-AMINO-2-MERCAPTOETHYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>GYC</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gx0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gx0 OCA], [https://pdbe.org/2gx0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gx0 RCSB], [https://www.ebi.ac.uk/pdbsum/2gx0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gx0 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q5TLG6_9CNID Q5TLG6_9CNID]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 35: / Line 37: @@
 __TOC__
 </StructureSection>
-[[Category: Echinophyllia sp. sc22]]
+[[Category: Echinophyllia sp. SC22]]
 [[Category: Large Structures]]
-[[Category: Hwang, K Y]]
+[[Category: Hwang KY]]
-[[Category: Nam, K H]]
+[[Category: Nam K-H]]
-[[Category: Park, S Y]]
+[[Category: Park S-Y]]
-[[Category: Sugiyama, K]]
+[[Category: Sugiyama K]]
-[[Category: B-can]]
-[[Category: Fluorescence]]
-[[Category: Luminescent protein]]
-[[Category: Photoswitching]]

2gx0

From Proteopedia

Revision as of 08:49, 25 October 2023

Crystal structural and functional analysis of GFP-like fluorescent protein

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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