This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

2htj

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Current revision

NMR structure of E.coli PapI

Structural highlights

2htj is a 1 chain structure with sequence from Escherichia coli. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

KS71A_ECOLX

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Pyelonephritis-associated pili (pap) allow uropathogenic Escherichia coli to bind to epithelial cells and play an important role in urinary tract infection. Expression of pap is controlled by a phase-variation mechanism, based on the two distinct heritable states that are the result of adenine N6-methylation in either of the two GATC sequences in its regulatory region. The methylation status of these two sequences is sensed by the action of two proteins, Lrp and PapI, and they play a central role in determining pap gene expression in both phase-ON and phase-OFF cells. We used modern NMR techniques to determine the solution structure and backbone dynamics of PapI. We found its overall fold resembles closely that of the winged helix-turn-helix family of DNA-binding proteins. We determined that PapI possesses its own DNA-binding activity, albeit non-sequence-specific, independent of Lrp. PapI appears to bind to DNA with a K(d) in the 10 microM range. Possible mechanisms by which PapI might participate in the regulation of the pap operon are discussed in light of these new findings.

Solution structure of Escherichia coli PapI, a key regulator of the pap pili phase variation.,Kawamura T, Le LU, Zhou H, Dahlquist FW J Mol Biol. 2007 Jan 26;365(4):1130-42. Epub 2006 Oct 25. PMID:17109885^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kawamura T, Le LU, Zhou H, Dahlquist FW. Solution structure of Escherichia coli PapI, a key regulator of the pap pili phase variation. J Mol Biol. 2007 Jan 26;365(4):1130-42. Epub 2006 Oct 25. PMID:17109885 doi:10.1016/j.jmb.2006.10.066

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2htj"

@@ Line 1: / Line 1: @@
 ==NMR structure of E.coli PapI==
-<StructureSection load='2htj' size='340' side='right'caption='[[2htj]], [[NMR_Ensembles_of_Models | 1 NMR models]]' scene=''>
+<StructureSection load='2htj' size='340' side='right'caption='[[2htj]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2htj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HTJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HTJ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2htj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HTJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HTJ FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2htj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2htj OCA], [https://pdbe.org/2htj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2htj RCSB], [https://www.ebi.ac.uk/pdbsum/2htj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2htj ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2htj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2htj OCA], [https://pdbe.org/2htj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2htj RCSB], [https://www.ebi.ac.uk/pdbsum/2htj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2htj ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/KS71A_ECOLX KS71A_ECOLX]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 29: / Line 32: @@
 __TOC__
 </StructureSection>
-[[Category: Bacillus coli migula 1895]]
+[[Category: Escherichia coli]]
 [[Category: Large Structures]]
-[[Category: Dahlquist, F W]]
+[[Category: Dahlquist FW]]
-[[Category: Kawamura, T]]
+[[Category: Kawamura T]]
-[[Category: Le, L U.K]]
+[[Category: Le LUK]]
-[[Category: Zhou, H]]
+[[Category: Zhou H]]
-[[Category: Pap pili]]
-[[Category: Transcription activator]]
-[[Category: Winged helix-turn-helix]]

2htj

From Proteopedia

Current revision

NMR structure of E.coli PapI

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox