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1g38

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{{STRUCTURE_1g38| PDB=1g38 | SCENE= }}
{{STRUCTURE_1g38| PDB=1g38 | SCENE= }}
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'''ADENINE-SPECIFIC METHYLTRANSFERASE M. TAQ I/DNA COMPLEX'''
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===ADENINE-SPECIFIC METHYLTRANSFERASE M. TAQ I/DNA COMPLEX===
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==Overview==
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The 2.0 A crystal structure of the N6-adenine DNA methyltransferase M.TaqI in complex with specific DNA and a nonreactive cofactor analog reveals a previously unrecognized stabilization of the extrahelical target base. To catalyze the transfer of the methyl group from the cofactor S-adenosyl-l-methionine to the 6-amino group of adenine within the double-stranded DNA sequence 5'-TCGA-3', the target nucleoside is rotated out of the DNA helix. Stabilization of the extrahelical conformation is achieved by DNA compression perpendicular to the DNA helix axis at the target base pair position and relocation of the partner base thymine in an interstrand pi-stacked position, where it would sterically overlap with an innerhelical target adenine. The extrahelical target adenine is specifically recognized in the active site, and the 6-amino group of adenine donates two hydrogen bonds to Asn 105 and Pro 106, which both belong to the conserved catalytic motif IV of N6-adenine DNA methyltransferases. These hydrogen bonds appear to increase the partial negative charge of the N6 atom of adenine and activate it for direct nucleophilic attack on the methyl group of the cofactor.
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(as it appears on PubMed at http://www.pubmed.gov), where 11175899 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11175899}}
==About this Structure==
==About this Structure==
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[[Category: Restriction system]]
[[Category: Restriction system]]
[[Category: Transferase]]
[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:05:00 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 04:20:43 2008''

Revision as of 01:20, 1 July 2008

Image:1g38.png

Template:STRUCTURE 1g38

ADENINE-SPECIFIC METHYLTRANSFERASE M. TAQ I/DNA COMPLEX

Template:ABSTRACT PUBMED 11175899

About this Structure

1G38 is a Single protein structure of sequence from Thermus aquaticus. Full crystallographic information is available from OCA.

Reference

Structure of the N6-adenine DNA methyltransferase M.TaqI in complex with DNA and a cofactor analog., Goedecke K, Pignot M, Goody RS, Scheidig AJ, Weinhold E, Nat Struct Biol. 2001 Feb;8(2):121-5. PMID:11175899

Page seeded by OCA on Tue Jul 1 04:20:43 2008

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