7wek
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of the mouse Wdr47 NTD in complex with the WBR motif form Camsap3.== | |
| + | <StructureSection load='7wek' size='340' side='right'caption='[[7wek]], [[Resolution|resolution]] 3.21Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7wek]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WEK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WEK FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wek FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wek OCA], [https://pdbe.org/7wek PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wek RCSB], [https://www.ebi.ac.uk/pdbsum/7wek PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wek ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/WDR47_MOUSE WDR47_MOUSE] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Calmodulin-regulated spectrin-associated proteins (Camsaps) bind to the N-terminal domain of WD40-repeat 47 (Wdr47-NTD; featured with a LisH-CTLH motif) to properly generate axonemal central-pair microtubules (CP-MTs) for the planar beat pattern of mammalian motile multicilia. The underlying molecular mechanism, however, remains unclear. Here, we determine the structures of apo-Wdr47-NTD and Wdr47-NTD in complex with a characteristic Wdr47-binding region (WBR) from Camsap3. Wdr47-NTD forms an intertwined dimer with a special cross-over region (COR) in addition to the canonical LisH and globular alpha-helical core (GAC). The basic WBR peptide adopts an alpha-helical conformation and anchors to a tailored acidic pocket embedded in the COR. Mutations in this target-binding pocket disrupt the interaction between Wdr47-NTD and Camsap3. Impairing Wdr47-Camsap interactions markedly reduces rescue effects of Wdr47 on CP-MTs and ciliary beat of Wdr47-deficient ependymal cells. Thus, Wdr47-NTD functions by recognizing a specific basic helical motif in Camsap proteins via its non-canonical COR, a target-binding site in LisH-CTLH-containing domains. | ||
| - | + | Intertwined Wdr47-NTD dimer recognizes a basic-helical motif in Camsap proteins for proper central-pair microtubule formation.,Ren J, Li D, Liu J, Liu H, Yan X, Zhu X, Feng W Cell Rep. 2022 Nov 8;41(6):111589. doi: 10.1016/j.celrep.2022.111589. PMID:36351391<ref>PMID:36351391</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 7wek" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Mus musculus]] | ||
| + | [[Category: Feng W]] | ||
| + | [[Category: Li D]] | ||
| + | [[Category: Ren JQ]] | ||
Revision as of 10:39, 30 November 2022
Crystal structure of the mouse Wdr47 NTD in complex with the WBR motif form Camsap3.
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Categories: Large Structures | Mus musculus | Feng W | Li D | Ren JQ
