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1g3v

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{{STRUCTURE_1g3v| PDB=1g3v | SCENE= }}
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'''CRYSTAL STRUCTURE OF NICKEL-D[CGTGTACACG]2'''
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===CRYSTAL STRUCTURE OF NICKEL-D[CGTGTACACG]2===
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==Overview==
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The aim of this study was to clarify whether Ni2+ ions could bind to guanine bases in a standard B-DNA duplex and eventually induce a B--&gt;Z transition. We have determined by X-ray crystallography at 3.1 A resolution the structure of the alternating deoxynucleotide d(CGTGTACACG), which contains both internal and terminal guanines. The duplex is in the B form. It is shown that nickel ions bind selectively to the N7 atom of guanine 10, which is in an extra-helical position, and guanine 2, which is in the terminal position of the duplex. It does not bind to guanine 4, which lies within a standard B-DNA tract. This simple but unambiguous result proves that nickel ions select between different guanines via steric accessibility. Guanine-Ni2+-guanine bridges among symmetry-related duplexes have also been found. These bridges may explain why Ni2+ ions may act either as a precipitant or a renaturing agent for DNA under certain conditions. The biochemical interaction of nickel with DNA can thus be related to its capacity to specifically bind to B-DNA regions with exposed guanines. Also, from the structural point of view, we have found a terminal cytosine, which forms a C.G:C reverse-Hoogsteen triple structure with a base pair of a neighbor duplex. This type of triplet is seldom found and is here described for the first time for a DNA structure.
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==About this Structure==
==About this Structure==
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[[Category: B-dna]]
[[Category: B-dna]]
[[Category: Nickel binding]]
[[Category: Nickel binding]]
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Revision as of 01:22, 1 July 2008

Image:1g3v.png

Template:STRUCTURE 1g3v

CRYSTAL STRUCTURE OF NICKEL-D[CGTGTACACG]2

Template:ABSTRACT PUBMED 11862555

About this Structure

Full crystallographic information is available from OCA.

Reference

Nickel-guanine interactions in DNA: crystal structure of nickel-d[CGTGTACACG]2., Abrescia NA, Huynh-Dinh T, Subirana JA, J Biol Inorg Chem. 2002 Jan;7(1-2):195-9. Epub 2001 Aug 25. PMID:11862555

Page seeded by OCA on Tue Jul 1 04:22:29 2008

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