1jqt
From Proteopedia
(Difference between revisions)
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1jqt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JQT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JQT FirstGlance]. <br> | <table><tr><td colspan='2'>[[1jqt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JQT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JQT FirstGlance]. <br> | ||
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 18Å</td></tr> |
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jqt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jqt OCA], [https://pdbe.org/1jqt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jqt RCSB], [https://www.ebi.ac.uk/pdbsum/1jqt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jqt ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jqt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jqt OCA], [https://pdbe.org/1jqt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jqt RCSB], [https://www.ebi.ac.uk/pdbsum/1jqt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jqt ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
| - | + | [https://www.uniprot.org/uniprot/RL11_THEMA RL11_THEMA] This protein binds directly to 23S ribosomal RNA. | |
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jqt ConSurf]. | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jqt ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | L11 protein is located at the base of the L7/L12 stalk of the 50 S subunit of the Escherichia coli ribosome. Because of the flexible nature of the region, recent X-ray crystallographic studies of the 50 S subunit failed to locate the N-terminal domain of the protein. We have determined the position of the complete L11 protein by comparing a three-dimensional cryo-EM reconstruction of the 70 S ribosome, isolated from a mutant lacking ribosomal protein L11, with the three-dimensional map of the wild-type ribosome. Fitting of the X-ray coordinates of L11-23 S RNA complex and EF-G into the cryo-EM maps combined with molecular modeling, reveals that, following EF-G-dependent GTP hydrolysis, domain V of EF-G intrudes into the cleft between the 23 S ribosomal RNA and the N-terminal domain of L11 (where the antibiotic thiostrepton binds), causing the N-terminal domain to move and thereby inducing the formation of the arc-like connection with the G' domain of EF-G. The results provide a new insight into the mechanism of EF-G-dependent translocation. | ||
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| - | Localization of L11 protein on the ribosome and elucidation of its involvement in EF-G-dependent translocation.,Agrawal RK, Linde J, Sengupta J, Nierhaus KH, Frank J J Mol Biol. 2001 Aug 24;311(4):777-87. PMID:11518530<ref>PMID:11518530</ref> | ||
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 1jqt" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
| - | *[[Ribosomal protein L11|Ribosomal protein L11]] | + | *[[Ribosomal protein L11 3D structures|Ribosomal protein L11 3D structures]] |
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__TOC__ | __TOC__ | ||
</SX> | </SX> | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Agrawal | + | [[Category: Agrawal RK]] |
| - | [[Category: Frank | + | [[Category: Frank J]] |
| - | [[Category: Linde | + | [[Category: Linde J]] |
| - | [[Category: Nierhaus | + | [[Category: Nierhaus KH]] |
| - | [[Category: Segupta | + | [[Category: Segupta J]] |
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Current revision
Fitting of L11 protein in the low resolution cryo-EM map of E.coli 70S ribosome
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