2bg9

From Proteopedia

(Difference between revisions)

Revision as of 11:21, 22 May 2024

REFINED STRUCTURE OF THE NICOTINIC ACETYLCHOLINE RECEPTOR AT 4A RESOLUTION.

2bg9, resolution 4.00Å

Structural highlights

2bg9 is a 5 chain structure with sequence from Torpedo marmorata. The November 2005 RCSB PDB Molecule of the Month feature on Acetylcholine Receptor by David S. Goodsell is 10.2210/rcsb_pdb/mom_2005_11. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 4Å
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ACHA_TORMA After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

Evolutionary Conservation

Checkto colour the structure by Evolutionary Conservation, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

We present a refined model of the membrane-associated Torpedo acetylcholine (ACh) receptor at 4A resolution. An improved experimental density map was obtained from 342 electron images of helical tubes, and the refined structure was derived to an R-factor of 36.7% (R(free) 37.9%) by standard crystallographic methods, after placing the densities corresponding to a single molecule into an artificial unit cell. The agreement between experimental and calculated phases along the helical layer-lines was used to monitor progress in the refinement and to give an independent measure of the accuracy. The atomic model allowed a detailed description of the whole receptor in the closed-channel form, including the ligand-binding and intracellular domains, which have not previously been interpreted at a chemical level. We confirm that the two ligand-binding alpha subunits have a different extended conformation from the three other subunits in the closed channel, and identify several interactions on both pairs of subunit interfaces, and within the alpha subunits, which may be responsible for their "distorted" structures. The ACh-coordinating amino acid side-chains of the alpha subunits are far apart in the closed channel, indicating that a localised rearrangement, involving closure of loops B and C around the bound ACh molecule, occurs upon activation. A comparison of the structure of the alpha subunit with that of AChBP having ligand present, suggests how the localised rearrangement overcomes the distortions and initiates the rotational movements associated with opening of the channel. Both vestibules of the channel are strongly electronegative, providing a cation-stabilising environment at either entrance of the membrane pore. Access to the pore on the intracellular side is further influenced by narrow lateral windows, which would be expected to screen out electrostatically ions of the wrong charge and size.

Refined structure of the nicotinic acetylcholine receptor at 4A resolution.,Unwin N J Mol Biol. 2005 Mar 4;346(4):967-89. Epub 2005 Jan 25. PMID:15701510^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Unwin N. Refined structure of the nicotinic acetylcholine receptor at 4A resolution. J Mol Biol. 2005 Mar 4;346(4):967-89. Epub 2005 Jan 25. PMID:15701510 doi:10.1016/j.jmb.2004.12.031

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2bg9"

Categories: Acetylcholine Receptor | Large Structures | RCSB PDB Molecule of the Month | Torpedo marmorata | Unwin N

@@ Line 4: / Line 4: @@
 == Structural highlights ==
 <table><tr><td colspan='2'>[[2bg9]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Torpedo_marmorata Torpedo marmorata]. The November 2005 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Acetylcholine Receptor''  by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2005_11 10.2210/rcsb_pdb/mom_2005_11]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BG9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BG9 FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1l4w|1l4w]], [[1ljz|1ljz]], [[1oed|1oed]], [[1i9b|1i9b]], [[1uv6|1uv6]], [[1f3r|1f3r]], [[1tor|1tor]], [[1tos|1tos]]</div></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4&#8491;</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bg9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bg9 OCA], [https://pdbe.org/2bg9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bg9 RCSB], [https://www.ebi.ac.uk/pdbsum/2bg9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bg9 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/ACHA_TORMA ACHA_TORMA]] After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
+[https://www.uniprot.org/uniprot/ACHA_TORMA ACHA_TORMA] After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 30: / Line 30: @@
 ==See Also==
+*[[Acetyl choline receptor 3D structures|Acetyl choline receptor 3D structures]]
+*[[Ionotropic receptors|Ionotropic receptors]]
 *[[Nicotinic Acetylcholine Receptor|Nicotinic Acetylcholine Receptor]]
 *[[5-hydroxytryptamine receptor|5-hydroxytryptamine receptor]]
@@ Line 40: / Line 42: @@
 [[Category: RCSB PDB Molecule of the Month]]
 [[Category: Torpedo marmorata]]
-[[Category: Unwin, N]]
+[[Category: Unwin N]]
-[[Category: Acetylcholine receptor]]
-[[Category: Ion channel]]
-[[Category: Ion channel-receptor complex]]
-[[Category: Ion channel/receptor]]
-[[Category: Ion transport]]
-[[Category: Postsynaptic membrane]]

2bg9

From Proteopedia

Revision as of 11:21, 22 May 2024

REFINED STRUCTURE OF THE NICOTINIC ACETYLCHOLINE RECEPTOR AT 4A RESOLUTION.

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox