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| - | [[Image:1g4h.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1g4h| PDB=1g4h | SCENE= }} | | {{STRUCTURE_1g4h| PDB=1g4h | SCENE= }} |
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| - | '''LINB COMPLEXED WITH BUTAN-1-OL'''
| + | ===LINB COMPLEXED WITH BUTAN-1-OL=== |
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| - | ==Overview==
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| - | The hydrolysis of haloalkanes to their corresponding alcohols and inorganic halides is catalyzed by alpha/beta-hydrolases called haloalkane dehalogenases. The study of haloalkane dehalogenases is vital for the development of these enzymes if they are to be utilized for bioremediation of organohalide-contaminated industrial waste. We report the kinetic and structural analysis of the haloalkane dehalogenase from Sphingomonas paucimobilis UT26 (LinB) in complex with each of 1,2-dichloroethane and 1,2-dichloropropane and the reaction product of 1-chlorobutane turnover. Activity studies showed very weak but detectable activity of LinB with 1,2-dichloroethane [0.012 nmol s(-1) (mg of enzyme)(-1)] and 1,2-dichloropropane [0.027 nmol s(-1) (mg of enzyme)(-1)]. These activities are much weaker compared, for example, to the activity of LinB with 1-chlorobutane [68.2 nmol s(-1) (mg of enzyme)(-1)]. Inhibition analysis reveals that both 1,2-dichloroethane and 1,2-dichloropropane act as simple competitive inhibitors of the substrate 1-chlorobutane and that 1,2-dichloroethane binds to LinB with lower affinity than 1,2-dichloropropane. Docking calculations on the enzyme in the absence of active site water molecules and halide ions confirm that these compounds could bind productively. However, when these moieties were included in the calculations, they bound in a manner similar to that observed in the crystal structure. These data provide an explanation for the low activity of LinB with small, chlorinated alkanes and show the importance of active site water molecules and reaction products in molecular docking. | + | The line below this paragraph, {{ABSTRACT_PUBMED_11939779}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 11939779 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_11939779}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Wilce, M C.J.]] | | [[Category: Wilce, M C.J.]] |
| | [[Category: Linb haloalkane dehalogenase halocarbon]] | | [[Category: Linb haloalkane dehalogenase halocarbon]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:07:45 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 04:24:08 2008'' |
Revision as of 01:24, 1 July 2008
Template:STRUCTURE 1g4h
LINB COMPLEXED WITH BUTAN-1-OL
Template:ABSTRACT PUBMED 11939779
About this Structure
1G4H is a Single protein structure of sequence from Sphingomonas paucimobilis. Full crystallographic information is available from OCA.
Reference
Exploring the structure and activity of haloalkane dehalogenase from Sphingomonas paucimobilis UT26: evidence for product- and water-mediated inhibition., Oakley AJ, Prokop Z, Bohac M, Kmunicek J, Jedlicka T, Monincova M, Kuta-Smatanova I, Nagata Y, Damborsky J, Wilce MC, Biochemistry. 2002 Apr 16;41(15):4847-55. PMID:11939779
Page seeded by OCA on Tue Jul 1 04:24:08 2008
Categories: Single protein | Sphingomonas paucimobilis | Bohac, M. | Damborsky, J. | Jedlicka, T. | Kmunicek, J. | Kuta-Smatanova, I. | Monincova, M. | Nagata, Y. | Oakley, A J. | Prokop, Z. | Wilce, M C.J. | Linb haloalkane dehalogenase halocarbon
