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1g58

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{{STRUCTURE_1g58| PDB=1g58 | SCENE= }}
{{STRUCTURE_1g58| PDB=1g58 | SCENE= }}
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'''CRYSTAL STRUCTURE OF 3,4-DIHYDROXY-2-BUTANONE 4-PHOSPHATE SYNTHASE GOLD DERIVATIVE'''
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===CRYSTAL STRUCTURE OF 3,4-DIHYDROXY-2-BUTANONE 4-PHOSPHATE SYNTHASE GOLD DERIVATIVE===
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==Overview==
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BACKGROUND: 3,4-Dihydroxy-2-butanone-4-phosphate synthase catalyzes a commitment step in the biosynthesis of riboflavin. On the enzyme, ribulose 5-phosphate is converted to 3,4-dihydroxy-2-butanone 4-phosphate and formate in steps involving enolization, ketonization, dehydration, skeleton rearrangement, and formate elimination. The enzyme is absent in humans and an attractive target for the discovery of antimicrobials for pathogens incapable of acquiring sufficient riboflavin from their hosts. The homodimer of 23 kDa subunits requires Mg(2+) for activity. RESULTS: The first three-dimensional structure of the enzyme was determined at 1.4 A resolution using the multiwavelength anomalous diffraction (MAD) method on Escherichia coli protein crystals containing gold. The protein consists of an alpha + beta fold having a complex linkage of beta strands. Intersubunit contacts are mediated by numerous hydrophobic interactions and three hydrogen bond networks. CONCLUSIONS: A proposed active site was identified on the basis of amino acid residues that are conserved among the enzyme from 19 species. There are two well-separated active sites per dimer, each of which comprise residues from both subunits. In addition to three arginines and two threonines, which may be used for recognizing the phosphate group of the substrate, the active site consists of three glutamates, two aspartates, two histidines, and a cysteine which may provide the means for general acid and base catalysis and for coordinating the Mg(2+) cofactor within the active site.
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(as it appears on PubMed at http://www.pubmed.gov), where 11342130 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11342130}}
==About this Structure==
==About this Structure==
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[[Category: Skeletal rearrangement]]
[[Category: Skeletal rearrangement]]
[[Category: Structure-based design]]
[[Category: Structure-based design]]
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Revision as of 01:26, 1 July 2008

Image:1g58.png

Template:STRUCTURE 1g58

CRYSTAL STRUCTURE OF 3,4-DIHYDROXY-2-BUTANONE 4-PHOSPHATE SYNTHASE GOLD DERIVATIVE

Template:ABSTRACT PUBMED 11342130

About this Structure

1G58 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystal structure of 3,4-dihydroxy-2-butanone 4-phosphate synthase of riboflavin biosynthesis., Liao DI, Calabrese JC, Wawrzak Z, Viitanen PV, Jordan DB, Structure. 2001 Jan 10;9(1):11-8. PMID:11342130

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