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| | <StructureSection load='6zui' size='340' side='right'caption='[[6zui]], [[Resolution|resolution]] 2.20Å' scene=''> | | <StructureSection load='6zui' size='340' side='right'caption='[[6zui]], [[Resolution|resolution]] 2.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6zui]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aeqvi Aeqvi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZUI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZUI FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6zui]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria] and [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZUI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZUI FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CR2:{(4Z)-2-(AMINOMETHYL)-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CR2</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.200082Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">nemR, ydhM, b1649, JW5874 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6100 AEQVI])</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CR2:{(4Z)-2-(AMINOMETHYL)-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CR2</scene></td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zui FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zui OCA], [https://pdbe.org/6zui PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zui RCSB], [https://www.ebi.ac.uk/pdbsum/6zui PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zui ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zui FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zui OCA], [https://pdbe.org/6zui PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zui RCSB], [https://www.ebi.ac.uk/pdbsum/6zui PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zui ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/NEMR_ECOLI NEMR_ECOLI]] Represses the transcription of the nemRA operon by binding to the nemR box.<ref>PMID:18567656</ref>
| + | [https://www.uniprot.org/uniprot/NEMR_ECOLI NEMR_ECOLI] Represses the transcription of the nemRA operon by binding to the nemR box.<ref>PMID:18567656</ref> [https://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Aeqvi]] | + | [[Category: Aequorea victoria]] |
| | + | [[Category: Escherichia coli K-12]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Messens, J]] | + | [[Category: Messens J]] |
| - | [[Category: Molle, I Van]]
| + | [[Category: Tossounian MA]] |
| - | [[Category: Tossounian, M A]] | + | [[Category: Van Molle I]] |
| - | [[Category: Biosensor]] | + | |
| - | [[Category: Cpyfp]]
| + | |
| - | [[Category: Fluorescent protein]]
| + | |
| - | [[Category: Hypohalous acid]]
| + | |
| - | [[Category: Yellow fluorescent protein]]
| + | |
| Structural highlights
Function
NEMR_ECOLI Represses the transcription of the nemRA operon by binding to the nemR box.[1] GFP_AEQVI Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
Publication Abstract from PubMed
The lack of tools to monitor the dynamics of (pseudo)hypohalous acids in live cells and tissues hinders a better understanding of inflammatory processes. Here we present a fluorescent genetically encoded biosensor, Hypocrates, for the visualization of (pseudo)hypohalous acids and their derivatives. Hypocrates consists of a circularly permuted yellow fluorescent protein integrated into the structure of the transcription repressor NemR from Escherichia coli. We show that Hypocrates is ratiometric, reversible, and responds to its analytes in the 10(6) M(-1)s(-1) range. Solving the Hypocrates X-ray structure provided insights into its sensing mechanism, allowing determination of the spatial organization in this circularly permuted fluorescent protein-based redox probe. We exemplify its applicability by imaging hypohalous stress in bacteria phagocytosed by primary neutrophils. Finally, we demonstrate that Hypocrates can be utilized in combination with HyPerRed for the simultaneous visualization of (pseudo)hypohalous acids and hydrogen peroxide dynamics in a zebrafish tail fin injury model.
Hypocrates is a genetically encoded fluorescent biosensor for (pseudo)hypohalous acids and their derivatives.,Kostyuk AI, Tossounian MA, Panova AS, Thauvin M, Raevskii RI, Ezerina D, Wahni K, Van Molle I, Sergeeva AD, Vertommen D, Gorokhovatsky AY, Baranov MS, Vriz S, Messens J, Bilan DS, Belousov VV Nat Commun. 2022 Jan 10;13(1):171. doi: 10.1038/s41467-021-27796-2. PMID:35013284[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Umezawa Y, Shimada T, Kori A, Yamada K, Ishihama A. The uncharacterized transcription factor YdhM is the regulator of the nemA gene, encoding N-ethylmaleimide reductase. J Bacteriol. 2008 Sep;190(17):5890-7. doi: 10.1128/JB.00459-08. Epub 2008 Jun 20. PMID:18567656 doi:http://dx.doi.org/10.1128/JB.00459-08
- ↑ Kostyuk AI, Tossounian MA, Panova AS, Thauvin M, Raevskii RI, Ezerina D, Wahni K, Van Molle I, Sergeeva AD, Vertommen D, Gorokhovatsky AY, Baranov MS, Vriz S, Messens J, Bilan DS, Belousov VV. Hypocrates is a genetically encoded fluorescent biosensor for (pseudo)hypohalous acids and their derivatives. Nat Commun. 2022 Jan 10;13(1):171. doi: 10.1038/s41467-021-27796-2. PMID:35013284 doi:http://dx.doi.org/10.1038/s41467-021-27796-2
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