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2jee

From Proteopedia

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Xray structure of E. coli YiiU

Structural highlights

2jee is a 4 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.8Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ZAPB_ECOLI Non-essential, abundant cell division factor that is required for proper Z-ring formation. It is recruited early to the divisome by direct interaction with FtsZ, stimulating Z-ring assembly and thereby promoting cell division earlier in the cell cycle. Its recruitment to the Z-ring requires functional FtsA or ZipA.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Formation of the Z ring is the first known event in bacterial cell division. However, it is not yet known how the assembly and contraction of the Z ring are regulated. Here, we identify a novel cell division factor ZapB in Escherichia coli that simultaneously stimulates Z ring assembly and cell division. Deletion of zapB resulted in delayed cell division and the formation of ectopic Z rings and spirals, whereas overexpression of ZapB resulted in nucleoid condensation and aberrant cell divisions. Localization of ZapB to the divisome depended on FtsZ but not FtsA, ZipA or FtsI, and ZapB interacted with FtsZ in a bacterial two-hybrid analysis. The simultaneous inactivation of FtsA and ZipA prevented Z ring assembly and ZapB localization. Time lapse microscopy showed that ZapB-GFP is present at mid-cell in a pattern very similar to that of FtsZ. Cells carrying a zapB deletion and the ftsZ84(ts) allele exhibited a synthetic sick phenotype and aberrant cell divisions. The crystal structure showed that ZapB exists as a dimer that is 100% coiled-coil. In vitro, ZapB self-assembled into long filaments and bundles. These results raise the possibility that ZapB stimulates Z ring formation directly via its capacity to self-assemble into larger structures.

Novel coiled-coil cell division factor ZapB stimulates Z ring assembly and cell division.,Ebersbach G, Galli E, Moller-Jensen J, Lowe J, Gerdes K Mol Microbiol. 2008 May;68(3):720-35. doi: 10.1111/j.1365-2958.2008.06190.x. PMID:18394147^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ebersbach G, Galli E, Moller-Jensen J, Lowe J, Gerdes K. Novel coiled-coil cell division factor ZapB stimulates Z ring assembly and cell division. Mol Microbiol. 2008 May;68(3):720-35. doi: 10.1111/j.1365-2958.2008.06190.x. PMID:18394147 doi:http://dx.doi.org/10.1111/j.1365-2958.2008.06190.x
↑ Ebersbach G, Galli E, Moller-Jensen J, Lowe J, Gerdes K. Novel coiled-coil cell division factor ZapB stimulates Z ring assembly and cell division. Mol Microbiol. 2008 May;68(3):720-35. doi: 10.1111/j.1365-2958.2008.06190.x. PMID:18394147 doi:http://dx.doi.org/10.1111/j.1365-2958.2008.06190.x

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2jee"

Categories: Escherichia coli | Large Structures | Gerdes K | Lowe J | Moller-Jensen J

@@ Line 3: / Line 3: @@
 <StructureSection load='2jee' size='340' side='right'caption='[[2jee]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2jee]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JEE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JEE FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2jee]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JEE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JEE FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jee FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jee OCA], [https://pdbe.org/2jee PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jee RCSB], [https://www.ebi.ac.uk/pdbsum/2jee PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jee ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jee FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jee OCA], [https://pdbe.org/2jee PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jee RCSB], [https://www.ebi.ac.uk/pdbsum/2jee PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jee ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/ZAPB_ECOLI ZAPB_ECOLI]] Non-essential, abundant cell division factor that is required for proper Z-ring formation. It is recruited early to the divisome by direct interaction with FtsZ, stimulating Z-ring assembly and thereby promoting cell division earlier in the cell cycle. Its recruitment to the Z-ring requires functional FtsA or ZipA.<ref>PMID:18394147</ref>
+[https://www.uniprot.org/uniprot/ZAPB_ECOLI ZAPB_ECOLI] Non-essential, abundant cell division factor that is required for proper Z-ring formation. It is recruited early to the divisome by direct interaction with FtsZ, stimulating Z-ring assembly and thereby promoting cell division earlier in the cell cycle. Its recruitment to the Z-ring requires functional FtsA or ZipA.<ref>PMID:18394147</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 34: / Line 35: @@
 __TOC__
 </StructureSection>
-[[Category: Bacillus coli migula 1895]]
+[[Category: Escherichia coli]]
 [[Category: Large Structures]]
-[[Category: Gerdes, K]]
+[[Category: Gerdes K]]
-[[Category: Lowe, J]]
+[[Category: Lowe J]]
-[[Category: Moller-Jensen, J]]
+[[Category: Moller-Jensen J]]
-[[Category: Cell cycle]]
-[[Category: Cell division]]
-[[Category: Coiled-coil]]
-[[Category: Ftsz]]
-[[Category: Hypothetical protein]]
-[[Category: Septum]]

2jee

From Proteopedia

Current revision

Xray structure of E. coli YiiU

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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