2k9o

From Proteopedia

(Difference between revisions)

Revision as of 18:07, 1 November 2023

Solution structure of Vm24 synthetic scorpion toxin

Structural highlights

2k9o is a 1 chain structure with sequence from Vaejovis smithi. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

KA231_VAEMS Voltage-gated potassium channel inhibitor. Selectively and irreversibly binds (K(d)=2.9 pM) and blocks hKv1.3/KCNA3 potassium channels of human T-lymphocytes. Weakly blocks hKCa3.1/KCNN4, mKv1.1/KCNA1, and hKv1.2/KCNA2 channels. In vivo, high doses (200 ug) produce no symptoms of intoxication when injected into mice.^[1] ^[2] ^[3]

Publication Abstract from PubMed

Animal venoms are rich sources of ligands for studying ion channels and other pharmacological targets. Proteomic analyses of the soluble venom from the Mexican scorpion Vaejovis mexicanus smithi showed that it contains more than 200 different components. Among them, a 36-residue peptide with a molecular mass of 3864 Da (named Vm24) was shown to be a potent blocker of Kv1.3 of human lymphocytes (K(d) approximately 3 pM). The three-dimensional solution structure of Vm24 was determined by nuclear magnetic resonance, showing the peptide folds into a distorted cystine-stabilized alpha/beta motif consisting of a single-turn alpha-helix and a three-stranded antiparallel beta-sheet, stabilized by four disulfide bridges. The disulfide pairs are formed between Cys6 and Cys26, Cys12 and Cys31, Cys16 and Cys33, and Cys21 and Cys36. Sequence analyses identified Vm24 as the first example of a new subfamily of alpha-type K(+) channel blockers (systematic number alpha-KTx 23.1). Comparison with other Kv1.3 blockers isolated from scorpions suggests a number of structural features that could explain the remarkable affinity and specificity of Vm24 toward Kv1.3 channels of lymphocytes.

Structure, function, and chemical synthesis of Vaejovis mexicanus peptide 24: a novel potent blocker of Kv1.3 potassium channels of human T lymphocytes.,Gurrola GB, Hernandez-Lopez RA, Rodriguez de la Vega RC, Varga Z, Batista CV, Salas-Castillo SP, Panyi G, del Rio-Portilla F, Possani LD Biochemistry. 2012 May 15;51(19):4049-61. Epub 2012 May 7. PMID:22540187^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Gurrola GB, Hernandez-Lopez RA, Rodriguez de la Vega RC, Varga Z, Batista CV, Salas-Castillo SP, Panyi G, del Rio-Portilla F, Possani LD. Structure, function, and chemical synthesis of Vaejovis mexicanus peptide 24: a novel potent blocker of Kv1.3 potassium channels of human T lymphocytes. Biochemistry. 2012 May 15;51(19):4049-61. Epub 2012 May 7. PMID:22540187 doi:http://dx.doi.org/10.1021/bi300060n
↑ Varga Z, Gurrola-Briones G, Papp F, Rodríguez de la Vega RC, Pedraza-Alva G, Tajhya RB, Gaspar R, Cardenas L, Rosenstein Y, Beeton C, Possani LD, Panyi G. Vm24, a natural immunosuppressive peptide, potently and selectively blocks Kv1.3 potassium channels of human T cells. Mol Pharmacol. 2012 Sep;82(3):372-82. PMID:22622363 doi:10.1124/mol.112.078006
↑ Gurrola GB, Hernandez-Lopez RA, Rodriguez de la Vega RC, Varga Z, Batista CV, Salas-Castillo SP, Panyi G, del Rio-Portilla F, Possani LD. Structure, function, and chemical synthesis of Vaejovis mexicanus peptide 24: a novel potent blocker of Kv1.3 potassium channels of human T lymphocytes. Biochemistry. 2012 May 15;51(19):4049-61. Epub 2012 May 7. PMID:22540187 doi:http://dx.doi.org/10.1021/bi300060n
↑ Gurrola GB, Hernandez-Lopez RA, Rodriguez de la Vega RC, Varga Z, Batista CV, Salas-Castillo SP, Panyi G, del Rio-Portilla F, Possani LD. Structure, function, and chemical synthesis of Vaejovis mexicanus peptide 24: a novel potent blocker of Kv1.3 potassium channels of human T lymphocytes. Biochemistry. 2012 May 15;51(19):4049-61. Epub 2012 May 7. PMID:22540187 doi:http://dx.doi.org/10.1021/bi300060n

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2k9o"

@@ Line 1: / Line 1: @@
 ==Solution structure of Vm24 synthetic scorpion toxin==
-<StructureSection load='2k9o' size='340' side='right'caption='[[2k9o]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='2k9o' size='340' side='right'caption='[[2k9o]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2k9o]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K9O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2K9O FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2k9o]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vaejovis_smithi Vaejovis smithi]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K9O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2K9O FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2k9o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k9o OCA], [https://pdbe.org/2k9o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2k9o RCSB], [https://www.ebi.ac.uk/pdbsum/2k9o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2k9o ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2k9o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k9o OCA], [https://pdbe.org/2k9o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2k9o RCSB], [https://www.ebi.ac.uk/pdbsum/2k9o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2k9o ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/KA231_VAEMS KA231_VAEMS] Voltage-gated potassium channel inhibitor. Selectively and irreversibly binds (K(d)=2.9 pM) and blocks hKv1.3/KCNA3 potassium channels of human T-lymphocytes. Weakly blocks hKCa3.1/KCNN4, mKv1.1/KCNA1, and hKv1.2/KCNA2 channels. In vivo, high doses (200 ug) produce no symptoms of intoxication when injected into mice.<ref>PMID:22540187</ref> <ref>PMID:22622363</ref> <ref>PMID:22540187</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 26: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Gurrola, G]]
+[[Category: Vaejovis smithi]]
-[[Category: Hernandez-Lopez, R]]
+[[Category: Gurrola G]]
-[[Category: Possani-Postay, L]]
+[[Category: Hernandez-Lopez R]]
-[[Category: Rio-Portilla, F del]]
+[[Category: Possani-Postay L]]
-[[Category: Alfa helix]]
+[[Category: Del Rio-Portilla F]]
-[[Category: Alfa/beta scaffold]]
-[[Category: Beta sheet]]
-[[Category: Scorpion k+ toxin]]
-[[Category: Toxin]]
-[[Category: Vm24]]

2k9o

From Proteopedia

Revision as of 18:07, 1 November 2023

Solution structure of Vm24 synthetic scorpion toxin

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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