2kd3

From Proteopedia

(Difference between revisions)

Current revision

NMR structure of the Wnt modulator protein Sclerostin

Structural highlights

2kd3 is a 1 chain structure with sequence from Mus musculus. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR, 15 models
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SOST_MOUSE Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Sclerostin has been identified as a negative regulator of bone growth. Initially it was considered that Sclerostin performs its regulatory function via acting as a modulator of bone morphogenetic proteins (BMPs) similar to known examples such as Noggin, Chordin, and members of the DAN family. Recent findings, however, show that Sclerostin interferes with the Wnt signaling pathway due to binding to the Wnt co-receptor LRP5 thereby modulating bone growth. As Sclerostin is exclusively produced by osteocytes located in bones, neutralization of its bone-inhibiting functions makes it a highly interesting target for an osteoanabolic therapeutic approach in diseases characterized by bone loss, such as osteoporosis. Despite the huge interest in Sclerostin inhibitors the molecular basis of its function and its interaction with components of the Wnt signaling cascade has remained unclear. Here, we present the NMR structure of murine Sclerostin providing the first insights how Sclerostin might bind to LRP5.

NMR structure of the Wnt modulator protein Sclerostin.,Weidauer SE, Schmieder P, Beerbaum M, Schmitz W, Oschkinat H, Mueller TD Biochem Biophys Res Commun. 2009 Feb 27;380(1):160-5. Epub 2009 Jan 21. PMID:19166819^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Lin C, Jiang X, Dai Z, Guo X, Weng T, Wang J, Li Y, Feng G, Gao X, He L. Sclerostin mediates bone response to mechanical unloading through antagonizing Wnt/beta-catenin signaling. J Bone Miner Res. 2009 Oct;24(10):1651-61. PMID:19419300 doi:10.1359/jbmr.090411
↑ Weidauer SE, Schmieder P, Beerbaum M, Schmitz W, Oschkinat H, Mueller TD. NMR structure of the Wnt modulator protein Sclerostin. Biochem Biophys Res Commun. 2009 Feb 27;380(1):160-5. Epub 2009 Jan 21. PMID:19166819 doi:10.1016/j.bbrc.2009.01.062

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2kd3"

Categories: Large Structures | Mus musculus | Mueller TD | Weidauer SE

@@ Line 1: / Line 1: @@
 ==NMR structure of the Wnt modulator protein Sclerostin==
-<StructureSection load='2kd3' size='340' side='right'caption='[[2kd3]], [[NMR_Ensembles_of_Models | 15 NMR models]]' scene=''>
+<StructureSection load='2kd3' size='340' side='right'caption='[[2kd3]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2kd3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KD3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KD3 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2kd3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KD3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KD3 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kd3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kd3 OCA], [https://pdbe.org/2kd3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kd3 RCSB], [https://www.ebi.ac.uk/pdbsum/2kd3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kd3 ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 15 models</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kd3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kd3 OCA], [https://pdbe.org/2kd3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kd3 RCSB], [https://www.ebi.ac.uk/pdbsum/2kd3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kd3 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/SOST_MOUSE SOST_MOUSE] Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.<ref>PMID:19419300</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 11: / Line 14: @@
   <jmolCheckbox>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kd/2kd3_consurf.spt"</scriptWhenChecked>
-    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
@@ Line 30: / Line 33: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Lk3 transgenic mice]]
+[[Category: Mus musculus]]
-[[Category: Mueller, T D]]
+[[Category: Mueller TD]]
-[[Category: Weidauer, S E]]
+[[Category: Weidauer SE]]
-[[Category: Antagonist of cytokine]]
-[[Category: Protein]]
-[[Category: Protein binding]]

2kd3

From Proteopedia

Current revision

NMR structure of the Wnt modulator protein Sclerostin

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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