1g8z

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1g8z.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:1g8z.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1g8z| PDB=1g8z | SCENE= }}
{{STRUCTURE_1g8z| PDB=1g8z | SCENE= }}
-
'''HIS57ALA MUTANT OF CHOLERA TOXIN B-PENATMER'''
+
===HIS57ALA MUTANT OF CHOLERA TOXIN B-PENATMER===
-
==Overview==
+
<!--
-
GM1-ganglioside receptor binding by the B subunit of cholera toxin (CtxB) is widely accepted to initiate toxin action by triggering uptake and delivery of the toxin A subunit into cells. More recently, GM1 binding by isolated CtxB, or the related B subunit of Escherichia coli heat-labile enterotoxin (EtxB), has been found to modulate leukocyte function, resulting in the down-regulation of proinflammatory immune responses that cause autoimmune disorders such as rheumatoid arthritis and diabetes. Here, we demonstrate that GM1 binding, contrary to expectation, is not sufficient to initiate toxin action. We report the engineering and crystallographic structure of a mutant cholera toxin, with a His to Ala substitution in the B subunit at position 57. Whereas the mutant retained pentameric stability and high affinity binding to GM1-ganglioside, it had lost its immunomodulatory activity and, when part of the holotoxin complex, exhibited ablated toxicity. The implications of these findings on the mode of action of cholera toxin are discussed.
+
The line below this paragraph, {{ABSTRACT_PUBMED_11447291}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 11447291 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_11447291}}
==About this Structure==
==About this Structure==
Line 28: Line 32:
[[Category: Rodigherio, C.]]
[[Category: Rodigherio, C.]]
[[Category: Toxin]]
[[Category: Toxin]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:17:38 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 04:54:29 2008''

Revision as of 01:54, 1 July 2008

Image:1g8z.png

Template:STRUCTURE 1g8z

HIS57ALA MUTANT OF CHOLERA TOXIN B-PENATMER

Template:ABSTRACT PUBMED 11447291

About this Structure

1G8Z is a Single protein structure of sequence from Vibrio cholerae. Full crystallographic information is available from OCA.

Reference

A mutant cholera toxin B subunit that binds GM1- ganglioside but lacks immunomodulatory or toxic activity., Aman AT, Fraser S, Merritt EA, Rodigherio C, Kenny M, Ahn M, Hol WG, Williams NA, Lencer WI, Hirst TR, Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8536-41. Epub 2001 Jul 10. PMID:11447291

Page seeded by OCA on Tue Jul 1 04:54:29 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1g8z"
Personal tools