7m6k

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==Crystal structure of the ARM domain from Drosophila SARM1 in complex with VMN==
==Crystal structure of the ARM domain from Drosophila SARM1 in complex with VMN==
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<StructureSection load='7m6k' size='340' side='right'caption='[[7m6k]]' scene=''>
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<StructureSection load='7m6k' size='340' side='right'caption='[[7m6k]], [[Resolution|resolution]] 1.69&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7M6K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7M6K FirstGlance]. <br>
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<table><tr><td colspan='2'>[[7m6k]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7M6K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7M6K FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7m6k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7m6k OCA], [https://pdbe.org/7m6k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7m6k RCSB], [https://www.ebi.ac.uk/pdbsum/7m6k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7m6k ProSAT]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.69&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=YRJ:3-({[(4-nitrophenyl)carbamoyl]amino}methyl)-1-(5-O-phosphono-beta-D-ribofuranosyl)pyridin-1-ium'>YRJ</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7m6k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7m6k OCA], [https://pdbe.org/7m6k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7m6k RCSB], [https://www.ebi.ac.uk/pdbsum/7m6k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7m6k ProSAT]</span></td></tr>
</table>
</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SARM1_DROME SARM1_DROME] NAD(+) hydrolase, which plays a key role in axonal degeneration following injury by regulating NAD(+) metabolism (PubMed:22678360, PubMed:28334607). Acts as a negative regulator of MYD88- and TRIF-dependent toll-like receptor signaling pathway by promoting Wallerian degeneration, an injury-induced form of programmed subcellular death which involves degeneration of an axon distal to the injury site (PubMed:22678360). Wallerian degeneration is triggered by NAD(+) depletion: in response to injury, it is activated and catalyzes cleavage of NAD(+) into ADP-D-ribose (ADPR), cyclic ADPR (cADPR) and nicotinamide; NAD(+) cleavage promoting axon destruction (PubMed:22678360, PubMed:28334607, PubMed:31439792). Involved in the down-regulation of the tracheal immune response to Gram-negative bacteria (PubMed:22022271). This is likely by mediating Tollo signaling in the tracheal epithelium (PubMed:22022271).<ref>PMID:22022271</ref> <ref>PMID:22678360</ref> <ref>PMID:28334607</ref> <ref>PMID:31439792</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Axon loss underlies symptom onset and progression in many neurodegenerative disorders. Axon degeneration in injury and disease is promoted by activation of the NAD-consuming enzyme SARM1. Here, we report a novel activator of SARM1, a metabolite of the pesticide and neurotoxin vacor. Removal of SARM1 completely rescues mouse neurons from vacor-induced neuron and axon death in vitro and in vivo. We present the crystal structure of the Drosophila SARM1 regulatory domain complexed with this activator, the vacor metabolite VMN, which as the most potent activator yet known is likely to support drug development for human SARM1 and NMNAT2 disorders. This study indicates the mechanism of neurotoxicity and pesticide action by vacor, raises important questions about other pyridines in wider use today, provides important new tools for drug discovery, and demonstrates that removing SARM1 can robustly block programmed axon death induced by toxicity as well as genetic mutation.
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Neurotoxin-mediated potent activation of the axon degeneration regulator SARM1.,Loreto A, Angeletti C, Gu W, Osborne A, Nieuwenhuis B, Gilley J, Merlini E, Arthur-Farraj P, Amici A, Luo Z, Hartley-Tassell L, Ve T, Desrochers LM, Wang Q, Kobe B, Orsomando G, Coleman MP Elife. 2021 Dec 6;10. pii: 72823. doi: 10.7554/eLife.72823. PMID:34870595<ref>PMID:34870595</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 7m6k" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[SARM1 3D structures|SARM1 3D structures]]
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== References ==
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<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Drosophila melanogaster]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Gu W]]
[[Category: Gu W]]
[[Category: Kobe B]]
[[Category: Kobe B]]
[[Category: Luo Z]]
[[Category: Luo Z]]

Revision as of 16:06, 18 October 2023

Crystal structure of the ARM domain from Drosophila SARM1 in complex with VMN

PDB ID 7m6k

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