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| | {{STRUCTURE_1g95| PDB=1g95 | SCENE= }} | | {{STRUCTURE_1g95| PDB=1g95 | SCENE= }} |
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| - | '''CRYSTAL STRUCTURE OF S.PNEUMONIAE GLMU, APO FORM'''
| + | ===CRYSTAL STRUCTURE OF S.PNEUMONIAE GLMU, APO FORM=== |
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| - | ==Overview==
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| - | N-Acetylglucosamine-1-phosphate uridyltransferase (GlmU) is an essential bacterial enzyme with both an acetyltransferase and a uridyltransferase activity which have been mapped to the C-terminal and N-terminal domains, respectively. GlmU performs the last two steps in the synthesis of UDP-N-acetylglucosamine (UDP-GlcNAc), which is an essential precursor in both the peptidoglycan and the lipopolysaccharide metabolic pathways. GlmU is therefore an attractive target for potential antibiotics. Knowledge of its three-dimensional structure would provide a basis for rational drug design. We have determined the crystal structures of Streptococcus pneumoniae GlmU (SpGlmU) in apo form at 2.33 A resolution, and in complex with UDP-N-acetyl glucosamine and the essential co-factor Mg(2+) at 1.96 A resolution. The protein structure consists of an N-terminal domain with an alpha/beta-fold, containing the uridyltransferase active site, and a C-terminal domain with a long left-handed beta-sheet helix (LbetaH) domain. An insertion loop containing the highly conserved sequence motif Asn-Tyr-Asp-Gly protrudes from the left-handed beta-sheet helix domain. In the crystal, S. pneumoniae GlmU forms exact trimers, mainly through contacts between left-handed beta-sheet helix domains. UDP-N-acetylglucosamine and Mg(2+) are bound at the uridyltransferase active site, which is in a closed form. We propose a uridyltransferase mechanism in which the activation energy of the double negatively charged phosphorane transition state is lowered by charge compensation of Mg(2+) and the side-chain of Lys22.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_11124906}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 11124906 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_11124906}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Trimer]] | | [[Category: Trimer]] |
| | [[Category: Uridyltransferase pyrophosphorylase]] | | [[Category: Uridyltransferase pyrophosphorylase]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:18:00 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 04:54:52 2008'' |
Revision as of 01:54, 1 July 2008
Template:STRUCTURE 1g95
CRYSTAL STRUCTURE OF S.PNEUMONIAE GLMU, APO FORM
Template:ABSTRACT PUBMED 11124906
About this Structure
1G95 is a Single protein structure of sequence from Streptococcus pneumoniae. Full crystallographic information is available from OCA.
Reference
Crystal structures of Streptococcus pneumoniae N-acetylglucosamine-1-phosphate uridyltransferase, GlmU, in apo form at 2.33 A resolution and in complex with UDP-N-acetylglucosamine and Mg(2+) at 1.96 A resolution., Kostrewa D, D'Arcy A, Takacs B, Kamber M, J Mol Biol. 2001 Jan 12;305(2):279-89. PMID:11124906
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