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7wam
From Proteopedia
(Difference between revisions)
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==Crystal structure of HaloTag complexed with VL1== | ==Crystal structure of HaloTag complexed with VL1== | ||
| - | <StructureSection load='7wam' size='340' side='right'caption='[[7wam]]' scene=''> | + | <StructureSection load='7wam' size='340' side='right'caption='[[7wam]], [[Resolution|resolution]] 1.49Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WAM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WAM FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7wam]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WAM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WAM FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wam FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wam OCA], [https://pdbe.org/7wam PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wam RCSB], [https://www.ebi.ac.uk/pdbsum/7wam PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wam ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=8MH:3-[6-(2-azanylhydrazinyl)-1,3-bis(oxidanylidene)benzo[de]isoquinolin-2-yl]-N-[2-(2-hexoxyethoxy)ethyl]propanamide'>8MH</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> |
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Haloalkane_dehalogenase Haloalkane dehalogenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.8.1.5 3.8.1.5] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wam FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wam OCA], [https://pdbe.org/7wam PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wam RCSB], [https://www.ebi.ac.uk/pdbsum/7wam PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wam ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | We developed a proximity photo-crosslinking method (Spotlight) with a 4-azido-N-ethyl-1,8-naphthalimide (AzNP) moiety that can be converted to reactive aryl nitrene species using ambient blue light-emitting diode light. Using an AzNP-conjugated HaloTag ligand (VL1), blue light-induced photo-crosslinked products of various HaloTag-conjugated proteins of interest were detected in subcellular spaces in live cells. Chemical or heat stress-induced dynamic changes in the proteome were also detected, and photo-crosslinking in the mouse brain tissue was enabled. Using Spotlight, we further identified the host interactome of SARS-CoV-2 nucleocapsid (N) protein, which is essential for viral genome assembly. Mass analysis of the VL1-crosslinked product of N-HaloTag in HEK293T cells showed that RNA-binding proteins in stress granules were exclusively enriched in the cross-linked samples. These results tell that our method can reveal the interactome of protein of interest within a short distance in live cells. | ||
| + | |||
| + | A chemical tool for blue light-inducible proximity photo-crosslinking in live cells.,Mishra PK, Kang MG, Lee H, Kim S, Choi S, Sharma N, Park CM, Ko J, Lee C, Seo JK, Rhee HW Chem Sci. 2021 Dec 10;13(4):955-966. doi: 10.1039/d1sc04871f. eCollection 2022, Jan 26. PMID:35211260<ref>PMID:35211260</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7wam" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Haloalkane dehalogenase]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Kang M]] | + | [[Category: Kang, M]] |
| - | [[Category: Lee C]] | + | [[Category: Lee, C]] |
| - | [[Category: Lee H]] | + | [[Category: Lee, H]] |
| - | [[Category: Pratyush M]] | + | [[Category: Pratyush, M]] |
| - | [[Category: Rhee H]] | + | [[Category: Rhee, H]] |
| + | [[Category: Halotag]] | ||
| + | [[Category: Hydrolase]] | ||
Revision as of 07:28, 9 March 2022
Crystal structure of HaloTag complexed with VL1
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Categories: Haloalkane dehalogenase | Large Structures | Kang, M | Lee, C | Lee, H | Pratyush, M | Rhee, H | Halotag | Hydrolase
