1g9i

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{{STRUCTURE_1g9i| PDB=1g9i | SCENE= }}
{{STRUCTURE_1g9i| PDB=1g9i | SCENE= }}
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'''CRYSTAL STRUCTURE OF BETA-TRYSIN COMPLEX IN CYCLOHEXANE'''
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===CRYSTAL STRUCTURE OF BETA-TRYSIN COMPLEX IN CYCLOHEXANE===
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==Overview==
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The active trypsin inhibiting component, SPC1, was obtained during the synthesis of a 22-residue peptide with three disulfide bridges according to the mimic mung bean Bowman-Birk type inhibitor. The K(i) value of SPC1 is 1.2x10(-7) M. In order to determine the topological structure of SPC1, X-ray diffraction studies were carried out on the complex of SPC1 with bovine beta-trypsin. Only the binding loop of SPC1 resolved at 2.2 A resolution due to conformational flexibility of the other residues [1]. The amino acid sequence was re-determined and electrospray mass spectroscopy was also performed to ensure that no cleaving occurred on SPC1 and the primary sequence of SPC1 is correct. Because the protein is more rigid in nonaqueous medium as has been proved by others [2], we treated the complex of SPC1 with neat cyclohexane and then subjected it to X-ray diffraction analysis, and the result showed that all the 22 residues of SPC1 were located in the electron density map. So the topological structure of SPC1 has been determined, suggesting that crystal treatment with cyclohexane may be used as a method to determine the conformation of the disordered regions in protein crystal structures.
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(as it appears on PubMed at http://www.pubmed.gov), where 11257512 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11257512}}
==About this Structure==
==About this Structure==
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[[Category: Organic media]]
[[Category: Organic media]]
[[Category: Trypsin-inhibitor complex]]
[[Category: Trypsin-inhibitor complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:18:55 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 04:56:48 2008''

Revision as of 01:56, 1 July 2008

Image:1g9i.png

Template:STRUCTURE 1g9i

CRYSTAL STRUCTURE OF BETA-TRYSIN COMPLEX IN CYCLOHEXANE

Template:ABSTRACT PUBMED 11257512

About this Structure

1G9I is a Protein complex structure of sequences from Bos taurus. Full crystallographic information is available from OCA.

Reference

X-Ray study on an artificial mung bean inhibitor complex with bovine beta-trypsin in neat cyclohexane., Zhu G, Huang Q, Zhu Y, Li Y, Chi C, Tang Y, Biochim Biophys Acta. 2001 Mar 9;1546(1):98-106. PMID:11257512

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