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3d8l

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Revision as of 17:29, 8 November 2023

Crystal structure of ORF12 from the lactococcus lactis bacteriophage p2

Structural highlights

3d8l is a 3 chain structure with sequence from Lactococcus virus P2. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.9Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GP12_BPLP2 Probable chaperone for the tape measure protein. Might help to maintain the tape measure protein in solution during tail assembly.^[1]

Publication Abstract from PubMed

We report here the characterization of the nonstructural protein ORF12 of the virulent lactococcal phage p2, which belongs to the Siphoviridae family. ORF12 was produced as a soluble protein, which forms large oligomers (6- to 15-mers) in solution. Using anti-ORF12 antibodies, we have confirmed that ORF12 is not found in the virion structure but is detected in the second half of the lytic cycle, indicating that it is a late-expressed protein. The structure of ORF12, solved by single anomalous diffraction and refined at 2.9-A resolution, revealed a previously unknown fold as well as the presence of a hydrophobic patch at its surface. Furthermore, crystal packing of ORF12 formed long spirals in which a hydrophobic, continuous crevice was identified. This crevice exhibited a repeated motif of aromatic residues, which coincided with the same repeated motif usually found in tape measure protein (TMP), predicted to form helices. A model of a complex between ORF12 and a repeated motif of the TMP of phage p2 (ORF14) was generated, in which the TMP helix fitted exquisitely in the crevice and the aromatic patches of ORF12. We suggest, therefore, that ORF12 might act as a chaperone for TMP hydrophobic repeats, maintaining TMP in solution during the tail assembly of the lactococcal siphophage p2.

Crystal structure of ORF12 from Lactococcus lactis phage p2 identifies a tape measure protein chaperone.,Siponen M, Sciara G, Villion M, Spinelli S, Lichiere J, Cambillau C, Moineau S, Campanacci V J Bacteriol. 2009 Feb;191(3):728-34. Epub 2008 Dec 1. PMID:19047351^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Siponen M, Sciara G, Villion M, Spinelli S, Lichiere J, Cambillau C, Moineau S, Campanacci V. Crystal structure of ORF12 from Lactococcus lactis phage p2 identifies a tape measure protein chaperone. J Bacteriol. 2009 Feb;191(3):728-34. Epub 2008 Dec 1. PMID:19047351 doi:10.1128/JB.01363-08
↑ Siponen M, Sciara G, Villion M, Spinelli S, Lichiere J, Cambillau C, Moineau S, Campanacci V. Crystal structure of ORF12 from Lactococcus lactis phage p2 identifies a tape measure protein chaperone. J Bacteriol. 2009 Feb;191(3):728-34. Epub 2008 Dec 1. PMID:19047351 doi:10.1128/JB.01363-08

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3d8l"

@@ Line 3: / Line 3: @@
 <StructureSection load='3d8l' size='340' side='right'caption='[[3d8l]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3d8l]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Bplp2 Bplp2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3D8L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3D8L FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3d8l]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactococcus_virus_P2 Lactococcus virus P2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3D8L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3D8L FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ORF12 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=254252 BPLP2])</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3d8l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3d8l OCA], [https://pdbe.org/3d8l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3d8l RCSB], [https://www.ebi.ac.uk/pdbsum/3d8l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3d8l ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/GP12_BPLP2 GP12_BPLP2] Probable chaperone for the tape measure protein. Might help to maintain the tape measure protein in solution during tail assembly.<ref>PMID:19047351</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 20: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Bplp2]]
+[[Category: Lactococcus virus P2]]
 [[Category: Large Structures]]
-[[Category: Cambillau, C]]
+[[Category: Cambillau C]]
-[[Category: Campanacci, V]]
+[[Category: Campanacci V]]
-[[Category: Lichiere, J]]
+[[Category: Lichiere J]]
-[[Category: Moineau, S]]
+[[Category: Moineau S]]
-[[Category: Siponen, M I]]
+[[Category: Siponen MI]]
-[[Category: Spinelli, S]]
+[[Category: Spinelli S]]
-[[Category: Lactococcal phage p2]]
-[[Category: Orf12]]
-[[Category: Viral protein]]

3d8l

From Proteopedia

Revision as of 17:29, 8 November 2023

Crystal structure of ORF12 from the lactococcus lactis bacteriophage p2

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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