A new inactive conformation of SARS-CoV-2 main protease
Emanuele Fornasier, Maria Ludovica Macchia, Gabriele Giachin, Alice Sosic,
Matteo Pavan, Mattia Sturlese, Cristiano Salata, Stefano Moro, Barbara Gatto,
Massimo Bellanda and Roberto Battistutta [1]
Molecular Tour
The SARS-CoV-2 main protease, Mpro or 3CLpro, is a cysteine peptidase involved in the processing of the two overlapping polyproteins pp1a and pp1ab, with the formation of individual mature non-structural proteins (nsp). Playing a pivotal role in genome replication and transcription, it is a validated antiviral drug target against Covid-19 pandemic. Mpro forms , the only enzymatically active form of the enzyme. (PDB entry 6y2e). Protomer A in blue-based colors, protomer B in yellow/red-based colors. In green the two oxyanion loops and the two catalytic cysteines 145. Each Mpro protomer is composed of ; the chymotrypsin- and 3C protease-like directly control the catalytic event. The substrate-binding site is between domains I and II, and, at variance with the classical catalytic triad of chymotrypsin-like proteases, SARS-CoV-2 Mpro has a catalytic dyad, consisting of . A structural element essential for the catalysis is the so-called (in green), comprising residues 138-145.
In this paper, we describe a new inactive structure of the main protease of SARS-CoV-2 (7nij). Movements in the substrate-binding region and near the catalytic site result in a significant reshaping of the reaction center. of Mpro: “active” in SARS-CoV-2 Mpro 6y2e in pink; “collapsed-inactive” in SARS-CoV Mpro 1uj1 chain B in magenta; “new-inactive” in SARS-CoV-2 Mpro 7nij in green. Consequently, the conformation adopted by residues 139-144 of the oxyanion loop is catalytically-incompetent. (white dashed lines). The side-chain of catalytic Cys145 has a double conformation. Phe140, Leu141 and Asn142 play a major role in the shift between the new inactive and active conformations. . The new-inactive oxyanion loop (7nij) is in green and the active loop (6y2e) is in pink. There are large movements (white dashed lines) for the side-chains of Asn142 and Phe140. In the new-inactive conformation, Asn142 moves from an exposed position, ASA 153.74 Å2, to a buried one, ASA 49.00 Å2 and Phe140 moves from a buried position, ASA 14.79 Å2, to an exposed one, ASA 143.29 Å2. Gly143-NH (G-NH) of the oxyanion hole, involved in the stabilization of the tetrahedral intermediate, moves 8.8 Å away.
The movements of the oxyanion loop and of the N- and C-termini result in the weakening of the dimeric architecture, as evidenced by the decreases in the interaction surface area and in the number of inter-protomer interactions. This novel conformation is relevant both for the comprehension of the mechanism of action of Mpro within the catalytic cycle and for the success of the structure-based drug design of anti-viral drugs.
Reshaping of the S1 and S2' subsites. Molecular dynamics modeling of the hypothetical interaction of new-inactive Mpro with substrates is shown. Putative interaction with the 11mer pseudo-substrate peptide from structure 2q6g:
Putative interaction with the acyl-intermediate of Mpro C-terminal autoprocessing site:
References
- ↑ Fornasier E, Macchia ML, Giachin G, Sosic A, Pavan M, Sturlese M, Salata C, Moro S, Gatto B, Bellanda M, Battistutta R. A new inactive conformation of SARS-CoV-2 main protease. Acta Crystallogr D Struct Biol. 2022 Mar 1;78(Pt 3):363-378. doi:, 10.1107/S2059798322000948. Epub 2022 Feb 21. PMID:35234150 doi:http://dx.doi.org/10.1107/S2059798322000948
