7qno
From Proteopedia
(Difference between revisions)
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==Crystal structure of ligand-free Danio rerio HDAC6 CD1 CD2== | ==Crystal structure of ligand-free Danio rerio HDAC6 CD1 CD2== | ||
| - | <StructureSection load='7qno' size='340' side='right'caption='[[7qno]]' scene=''> | + | <StructureSection load='7qno' size='340' side='right'caption='[[7qno]], [[Resolution|resolution]] 2.38Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QNO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QNO FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7qno]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Danio_rerio Danio rerio]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QNO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QNO FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qno FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qno OCA], [https://pdbe.org/7qno PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qno RCSB], [https://www.ebi.ac.uk/pdbsum/7qno PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qno ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=PRO:PROLINE'>PRO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qno FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qno OCA], [https://pdbe.org/7qno PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qno RCSB], [https://www.ebi.ac.uk/pdbsum/7qno PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qno ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/F8W4B7_DANRE F8W4B7_DANRE] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Histone deacetylase 6 (HDAC6) is an atypical lysine deacetylase with tandem catalytic domains and an ubiquitin-binding zinc finger domain. HDAC6 is involved in various biological processes, such as cell motility or stress responses, and has been implicated in pathologies ranging from cancer to neurodegeneration. Due to this broad range of functions, there has been considerable interest in developing HDAC6-specific small molecule inhibitors, several of which are already available. The crystal structure of the tandem catalytic domains of zebrafish HDAC6 has revealed an arrangement with twofold symmetry and extensive surface interaction between the catalytic domains. Further dissection of the biochemical properties of HDAC6 and the development of novel inhibitors will benefit from being able to routinely express high-quality protein. We present here our optimized protocol for expression and crystallization of the zebrafish tandem catalytic domains. | ||
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| + | Expression and Crystallization of HDAC6 Tandem Catalytic Domains.,Langousis G, Sanchez J, Kempf G, Matthias P Methods Mol Biol. 2023;2589:467-480. doi: 10.1007/978-1-0716-2788-4_30. PMID:36255643<ref>PMID:36255643</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7qno" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Danio rerio]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Kempf G]] | [[Category: Kempf G]] | ||
Revision as of 10:24, 24 November 2022
Crystal structure of ligand-free Danio rerio HDAC6 CD1 CD2
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