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1gc2

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{{STRUCTURE_1gc2| PDB=1gc2 | SCENE= }}
{{STRUCTURE_1gc2| PDB=1gc2 | SCENE= }}
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'''CRYSTAL STRUCTURE OF THE PYRIDOXAL-5'-PHOSPHATE DEPENDENT L-METHIONINE GAMMA-LYASE FROM PSEUDOMONAS PUTIDA'''
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===CRYSTAL STRUCTURE OF THE PYRIDOXAL-5'-PHOSPHATE DEPENDENT L-METHIONINE GAMMA-LYASE FROM PSEUDOMONAS PUTIDA===
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==Overview==
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L-Methionine gamma-lyase (MGL) catalyzes the pyridoxal 5'-phosphate (PLP) dependent alpha,gamma-elimination of L-methionine. We have determined two crystal structures of MGL from Pseudomonas putida using MAD (multiwavelength anomalous diffraction) and molecular replacement methods. The structures have been refined to an R-factor of 21.1% at 2.0 and 1.7 A resolution using synchrotron radiation diffraction data. A homotetramer with 222 symmetry is built up by non-crystallographic symmetry. Two monomers associate to build the active dimer. The spatial fold of subunits, with three functionally distinct domains and their quarternary arrangement, is similar to those of L-cystathionine beta-lyase and L-cystathionine gamma-synthase from Escherichia coli.
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{{ABSTRACT_PUBMED_10965031}}
==About this Structure==
==About this Structure==
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[[Category: Yamamoto, M.]]
[[Category: Yamamoto, M.]]
[[Category: Pyridoxal-5'-phosphate]]
[[Category: Pyridoxal-5'-phosphate]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:23:55 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 05:03:33 2008''

Revision as of 02:03, 1 July 2008

Image:1gc2.png

Template:STRUCTURE 1gc2

CRYSTAL STRUCTURE OF THE PYRIDOXAL-5'-PHOSPHATE DEPENDENT L-METHIONINE GAMMA-LYASE FROM PSEUDOMONAS PUTIDA

Template:ABSTRACT PUBMED 10965031

About this Structure

1GC2 is a Single protein structure of sequence from Pseudomonas putida. Full crystallographic information is available from OCA.

Reference

Crystal structure of the pyridoxal 5'-phosphate dependent L-methionine gamma-lyase from Pseudomonas putida., Motoshima H, Inagaki K, Kumasaka T, Furuichi M, Inoue H, Tamura T, Esaki N, Soda K, Tanaka N, Yamamoto M, Tanaka H, J Biochem. 2000 Sep;128(3):349-54. PMID:10965031

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