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| | <StructureSection load='3e1z' size='340' side='right'caption='[[3e1z]], [[Resolution|resolution]] 1.86Å' scene=''> | | <StructureSection load='3e1z' size='340' side='right'caption='[[3e1z]], [[Resolution|resolution]] 1.86Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3e1z]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Carica_papaya Carica papaya] and [https://en.wikipedia.org/wiki/Trycr Trycr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E1Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3E1Z FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3e1z]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Carica_papaya Carica papaya] and [https://en.wikipedia.org/wiki/Trypanosoma_cruzi Trypanosoma cruzi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E1Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3E1Z FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.86Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1khq|1khq]], [[2nqd|2nqd]], [[3cbj|3cbj]], [[3cbk|3cbk]], [[2oul|2oul]], [[1me3|1me3]]</div></td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cha ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5693 TRYCR])</td></tr> | + | |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Papain Papain], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.2 3.4.22.2] </span></td></tr> | + | |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3e1z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3e1z OCA], [https://pdbe.org/3e1z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3e1z RCSB], [https://www.ebi.ac.uk/pdbsum/3e1z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3e1z ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3e1z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3e1z OCA], [https://pdbe.org/3e1z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3e1z RCSB], [https://www.ebi.ac.uk/pdbsum/3e1z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3e1z ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/CHAG_TRYCR CHAG_TRYCR] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | [[Category: Carica papaya]] | | [[Category: Carica papaya]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Papain]] | + | [[Category: Trypanosoma cruzi]] |
| - | [[Category: Trycr]]
| + | [[Category: Abrahamson M]] |
| - | [[Category: Abrahamson, M]] | + | [[Category: Bujacz A]] |
| - | [[Category: Bujacz, A]] | + | [[Category: Bujacz G]] |
| - | [[Category: Bujacz, G]] | + | [[Category: Jaskolski M]] |
| - | [[Category: Jaskolski, M]] | + | [[Category: Ljunggren A]] |
| - | [[Category: Ljunggren, A]] | + | [[Category: Redzynia I]] |
| - | [[Category: Redzynia, I]] | + | |
| - | [[Category: Allergen]]
| + | |
| - | [[Category: Chagas disease]]
| + | |
| - | [[Category: Chagasin-papain complex]]
| + | |
| - | [[Category: Cysteine proteinase]]
| + | |
| - | [[Category: Cytoplasmic vesicle]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Hydrolase inhibitor-hydrolase complex]]
| + | |
| - | [[Category: Protease]]
| + | |
| - | [[Category: Protease inhibitor]]
| + | |
| - | [[Category: Protein inhibitor]]
| + | |
| - | [[Category: Thiol protease]]
| + | |
| - | [[Category: Thiol protease inhibitor]]
| + | |
| - | [[Category: Zymogen]]
| + | |
| Structural highlights
Function
CHAG_TRYCR
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
A complex of chagasin, a protein inhibitor from Trypanosoma cruzi, and papain, a classic family C1 cysteine protease, has been crystallized. Kinetic studies revealed that inactivation of papain by chagasin is very fast (k(on) = 1.5 x 10(6) M(-1) x s(-1)), and results in the formation of a very tight, reversible complex (K(i) = 36 pM), with similar or better rate and equilibrium constants than those for cathepsins L and B. The high-resolution crystal structure shows an inhibitory wedge comprising three loops, which forms a number of contacts responsible for the high-affinity binding. Comparison with the structure of papain in complex with human cystatin B reveals that, despite entirely different folding, the two inhibitors utilize very similar atomic interactions, leading to essentially identical affinities for the enzyme. Comparisons of the chagasin-papain complex with high-resolution structures of chagasin in complexes with cathepsin L, cathepsin B and falcipain allowed the creation of a consensus map of the structural features that are important for efficient inhibition of papain-like enzymes. The comparisons also revealed a number of unique interactions that can be used to design enzyme-specific inhibitors. As papain exhibits high structural similarity to the catalytic domain of the T. cruzi enzyme cruzipain, the present chagasin-papain complex provides a reliable model of chagasin-cruzipain interactions. Such information, coupled with our identification of specificity-conferring interactions, should be important for the development of drugs for treatment of the devastating Chagas disease caused by this parasite.
Crystal structure of the parasite inhibitor chagasin in complex with papain allows identification of structural requirements for broad reactivity and specificity determinants for target proteases.,Redzynia I, Ljunggren A, Bujacz A, Abrahamson M, Jaskolski M, Bujacz G FEBS J. 2009 Feb;276(3):793-806. PMID:19143838[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Redzynia I, Ljunggren A, Bujacz A, Abrahamson M, Jaskolski M, Bujacz G. Crystal structure of the parasite inhibitor chagasin in complex with papain allows identification of structural requirements for broad reactivity and specificity determinants for target proteases. FEBS J. 2009 Feb;276(3):793-806. PMID:19143838 doi:10.1111/j.1742-4658.2008.06824.x
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