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1gco

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[[Image:1gco.gif|left|200px]]
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==Overview==
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{{ABSTRACT_11173533}}
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The crystal structure of glucose dehydrogenase (GlcDH) from Bacillus megaterium IWG3 has been determined to an R-factor of 17.9% at 1.7 A resolution. The enzyme consists of four identical subunits, which are similar to those of other short-chain reductases/dehydrogenases (SDRs) in their overall folding and subunit architecture, although cofactor binding sites and subunit interactions differ. Whereas a pair of basic residues is well conserved among NADP(+)-preferring SDRs, only Arg39 was found around the adenine ribose moiety of GlcDH. This suggests that one basic amino acid is enough to determine the coenzyme specificity. The four subunits are interrelated by three mutually perpendicular diad axes (P, Q, and R). While subunit interactions through the P-axis for GlcDH are not so different from those of the other SDRs, those through the Q-axis differ significantly. GlcDH was found to have weaker hydrophobic interactions in the Q-interface. Moreover, GlcDH lacks the salt bridge that stabilizes the subunit interaction in the Q-interface in the other SDRs. Hydrogen bonds between Q-axis related subunits are also less common than in the other SDRs. The GlcDH tetramer dissociates into inactive monomers at pH 9.0, which can be attributed mainly to the weakness of the Q-axis interface.
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==About this Structure==
==About this Structure==
1GCO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_megaterium Bacillus megaterium]. The following page contains interesting information on the relation of 1GCO with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb77_1.html Glucose Oxidase]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GCO OCA].
1GCO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_megaterium Bacillus megaterium]. The following page contains interesting information on the relation of 1GCO with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb77_1.html Glucose Oxidase]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GCO OCA].
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==Reference==
 
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Crystal structure of glucose dehydrogenase from Bacillus megaterium IWG3 at 1.7 A resolution., Yamamoto K, Kurisu G, Kusunoki M, Tabata S, Urabe I, Osaki S, J Biochem. 2001 Feb;129(2):303-12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11173533 11173533]
 
[[Category: Bacillus megaterium]]
[[Category: Bacillus megaterium]]
[[Category: Glucose 1-dehydrogenase]]
[[Category: Glucose 1-dehydrogenase]]
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[[Category: Oxidoreductase]]
[[Category: Oxidoreductase]]
[[Category: Short-chain dehydrogenase/reductase]]
[[Category: Short-chain dehydrogenase/reductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:25:14 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 26 17:24:54 2008''

Revision as of 14:24, 26 June 2008

Image:1gco.png

Template:STRUCTURE 1gco

CRYSTAL STRUCTURE OF GLUCOSE DEHYDROGENASE COMPLEXED WITH NAD+


Template:ABSTRACT 11173533

About this Structure

1GCO is a Single protein structure of sequence from Bacillus megaterium. The following page contains interesting information on the relation of 1GCO with [Glucose Oxidase]. Full crystallographic information is available from OCA.

Page seeded by OCA on Thu Jun 26 17:24:54 2008

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