This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

7vw0

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Current revision

Structure of a dimeric periplasmic protein

Structural highlights

7vw0 is a 2 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.447Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

V5J1S8_ECOLX

Publication Abstract from PubMed

Bacteria maintain copper balance by various copper response mechanisms. A plasmid gene encoding a methionine rich protein targeted to periplasm is adjacent to the sil operon that confers heavy metal resistance. However, the gene product Orf91 has not been characterized before. Using X-ray crystallography, we solved the structures of Orf91 in apo, cuprous ion-bound, and cupric ion-bound forms. An Orf91 protomer consists of three helices of which the C-terminal two helices belong to domain of unknown function 305 (DUF305), and two Orf91s dimerize into a six-helical bundle. The MxxHH motif specific for DUF305 is critical for cuprous ion binding, and the MxxMxxMHxxMM motif in the N-terminal helix contributes to cupric ion binding. The first histidine of MxxHH shows alternative conformations related to the redox state of copper ion. We suggest that Orf91 is an adaptable copper sponge in the periplasmic space.

Structural basis of copper binding by a dimeric periplasmic protein forming a six-helical bundle.,Yang J, Gao M, Wang J, He C, Wang X, Liu L J Inorg Biochem. 2022 Jan 19;229:111728. doi: 10.1016/j.jinorgbio.2022.111728. PMID:35066349^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Yang J, Gao M, Wang J, He C, Wang X, Liu L. Structural basis of copper binding by a dimeric periplasmic protein forming a six-helical bundle. J Inorg Biochem. 2022 Jan 19;229:111728. doi: 10.1016/j.jinorgbio.2022.111728. PMID:35066349 doi:http://dx.doi.org/10.1016/j.jinorgbio.2022.111728

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/7vw0"

Categories: Escherichia coli | Large Structures | Liu L | Yang J

@@ Line 3: / Line 3: @@
 <StructureSection load='7vw0' size='340' side='right'caption='[[7vw0]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[7vw0]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VW0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VW0 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[7vw0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VW0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VW0 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vw0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vw0 OCA], [https://pdbe.org/7vw0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vw0 RCSB], [https://www.ebi.ac.uk/pdbsum/7vw0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vw0 ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.447&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vw0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vw0 OCA], [https://pdbe.org/7vw0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vw0 RCSB], [https://www.ebi.ac.uk/pdbsum/7vw0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vw0 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/V5J1S8_ECOLX V5J1S8_ECOLX]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 19: / Line 22: @@
 __TOC__
 </StructureSection>
+[[Category: Escherichia coli]]
 [[Category: Large Structures]]
-[[Category: Liu, L]]
+[[Category: Liu L]]
-[[Category: Yang, J]]
+[[Category: Yang J]]
-[[Category: Copper binding protein]]
-[[Category: Metal binding protein]]
-[[Category: Metal homeostasis]]

7vw0

From Proteopedia

Current revision

Structure of a dimeric periplasmic protein

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox