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2hsl

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Revision as of 11:50, 22 November 2023

NMR structure of 13mer duplex DNA containing an abasic site, averaged structure (alpha anomer)

Structural highlights

2hsl is a 2 chain structure with sequence from Synthetic construct. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

A 4'-oxidized abasic site (X) has been synthesized in a defined duplex DNA sequence, 5'-d(CCAAAGXACCGGG)-3'/3'-d(GGTTTCATGGCCC)-5' (1). Its structure has been determined by two-dimensional NMR methods, molecular modeling, and molecular dynamics simulations. 1 is globally B-form with the base (A) opposite X intrahelical and well-stacked. Only the alpha anomer of X is observed, and the abasic site deoxyribose is largely intrahelical. These results are compared with a normal abasic site (Y) in the same sequence context (2). Y is composed of a 60:40 mixture of alpha and beta anomers (2alpha and 2beta). In both 2alpha and 2beta, the base (A) opposite Y is intrahelical and well-stacked and the abasic site deoxyribose is predominantly extrahelical, consistent with the reported structures of the normal abasic site in a similar sequence context [Hoehn, S. T., Turner, C. J., and Stubbe, J. (2001) Nucleic Acids Res. 29, 3413-3423]. Molecular dynamics simulations reveal that the normal abasic site appears to be conformationally more flexible than the 4'-oxidized abasic site. The importance of the structure and flexibility of the abasic site in the recognition by the DNA repair enzyme Ape1 is discussed.

Nuclear magnetic resonance structural studies and molecular modeling of duplex DNA containing normal and 4'-oxidized abasic sites.,Chen J, Dupradeau FY, Case DA, Turner CJ, Stubbe J Biochemistry. 2007 Mar 20;46(11):3096-107. Epub 2007 Feb 27. PMID:17323932^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Chen J, Dupradeau FY, Case DA, Turner CJ, Stubbe J. Nuclear magnetic resonance structural studies and molecular modeling of duplex DNA containing normal and 4'-oxidized abasic sites. Biochemistry. 2007 Mar 20;46(11):3096-107. Epub 2007 Feb 27. PMID:17323932 doi:10.1021/bi6024269

1 Structural highlights
2 Publication Abstract from PubMed
3 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2hsl"

@@ Line 1: / Line 1: @@
 ==NMR structure of 13mer duplex DNA containing an abasic site, averaged structure (alpha anomer)==
-<StructureSection load='2hsl' size='340' side='right'caption='[[2hsl]], [[NMR_Ensembles_of_Models | 1 NMR models]]' scene=''>
+<StructureSection load='2hsl' size='340' side='right'caption='[[2hsl]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2hsl]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HSL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HSL FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2hsl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HSL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HSL FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ORP:2-DEOXY-5-PHOSPHONO-RIBOSE'>ORP</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2hsk|2hsk]], [[2hou|2hou]], [[2hpx|2hpx]], [[2hsr|2hsr]], [[2hss|2hss]]</div></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ORP:2-DEOXY-5-PHOSPHONO-RIBOSE'>ORP</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hsl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hsl OCA], [https://pdbe.org/2hsl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hsl RCSB], [https://www.ebi.ac.uk/pdbsum/2hsl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hsl ProSAT]</span></td></tr>
 </table>
@@ Line 22: / Line 22: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Case, D A]]
+[[Category: Synthetic construct]]
-[[Category: Chen, J]]
+[[Category: Case DA]]
-[[Category: Dupradeau, F Y]]
+[[Category: Chen J]]
-[[Category: Stubbe, J]]
+[[Category: Dupradeau FY]]
-[[Category: Turner, C J]]
+[[Category: Stubbe J]]
-[[Category: Abasic site]]
+[[Category: Turner CJ]]
-[[Category: Ape1]]
-[[Category: Base excision repair]]
-[[Category: Dna]]
-[[Category: Dna lesion]]

2hsl

From Proteopedia

Revision as of 11:50, 22 November 2023

NMR structure of 13mer duplex DNA containing an abasic site, averaged structure (alpha anomer)

Structural highlights

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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