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| | <StructureSection load='3e85' size='340' side='right'caption='[[3e85]], [[Resolution|resolution]] 1.95Å' scene=''> | | <StructureSection load='3e85' size='340' side='right'caption='[[3e85]], [[Resolution|resolution]] 1.95Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3e85]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/European_yellow_lupine European yellow lupine]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E85 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3E85 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3e85]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lupinus_luteus Lupinus luteus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E85 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3E85 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BSU:1,3-DIPHENYLUREA'>BSU</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2qim|2qim]], [[1xdf|1xdf]], [[1icx|1icx]], [[1ifv|1ifv]], [[2flh|2flh]], [[1bv1|1bv1]]</div></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BSU:1,3-DIPHENYLUREA'>BSU</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pr10.2b, Ypr10.2b ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3873 European yellow lupine])</td></tr>
| + | |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3e85 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3e85 OCA], [https://pdbe.org/3e85 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3e85 RCSB], [https://www.ebi.ac.uk/pdbsum/3e85 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3e85 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3e85 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3e85 OCA], [https://pdbe.org/3e85 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3e85 RCSB], [https://www.ebi.ac.uk/pdbsum/3e85 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3e85 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/P102B_LUPLU P102B_LUPLU] Class II ribonuclease (RNase) (By similarity). Binds to several cytokinins including natural adenine-type (e.g. trans-zeatin and kinetin) and artificial urea-type (e.g. N,N'-diphenylurea and N-phenyl-N'-(2-chloro-4-pyridyl)urea) hormones (PubMed:18406424, PubMed:19220853, PubMed:29630775). Interacts with melatonin (PubMed:29630775).[UniProtKB:P52779]<ref>PMID:18406424</ref> <ref>PMID:19220853</ref> <ref>PMID:29630775</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: European yellow lupine]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Bujacz, A]] | + | [[Category: Lupinus luteus]] |
| - | [[Category: Bujacz, G]] | + | [[Category: Bujacz A]] |
| - | [[Category: Fernandes, H C]] | + | [[Category: Bujacz G]] |
| - | [[Category: Jaskolski, M]] | + | [[Category: Fernandes HC]] |
| - | [[Category: Sikorski, M M]] | + | [[Category: Jaskolski M]] |
| - | [[Category: Cytokinin]] | + | [[Category: Sikorski MM]] |
| - | [[Category: Diphenylurea]]
| + | |
| - | [[Category: Pathogenesis-related protein]]
| + | |
| - | [[Category: Plant defense]]
| + | |
| - | [[Category: Plant hormone]]
| + | |
| - | [[Category: Plant pr-10 protein]]
| + | |
| - | [[Category: Plant protein]]
| + | |
| - | [[Category: Yellow lupine]]
| + | |
| Structural highlights
Function
P102B_LUPLU Class II ribonuclease (RNase) (By similarity). Binds to several cytokinins including natural adenine-type (e.g. trans-zeatin and kinetin) and artificial urea-type (e.g. N,N'-diphenylurea and N-phenyl-N'-(2-chloro-4-pyridyl)urea) hormones (PubMed:18406424, PubMed:19220853, PubMed:29630775). Interacts with melatonin (PubMed:29630775).[UniProtKB:P52779][1] [2] [3]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Plant pathogenesis-related (PR) proteins of class 10 are the only group among the 17 PR protein families that are intracellular and cytosolic. Sequence conservation and the wide distribution of PR-10 proteins throughout the plant kingdom are an indication of an indispensable function in plants, but their true biological role remains obscure. Crystal and solution structures for several homologues have shown a similar overall fold with a vast internal cavity which, together with structural similarities to the steroidogenic acute regulatory protein-related lipid transfer domain and cytokinin-specific binding proteins, strongly indicate a ligand-binding role for the PR-10 proteins. This article describes the structure of a complex between a classic PR-10 protein [Lupinus luteus (yellow lupine) PR-10 protein of subclass 2, LlPR-10.2B] and N,N'-diphenylurea, a synthetic cytokinin. Synthetic cytokinins have been shown in various bioassays to exhibit activity similar to that of natural cytokinins. The present 1.95 A resolution crystallographic model reveals four N,N'-diphenylurea molecules in the hydrophobic cavity of the protein and a degree of conformational changes accompanying ligand binding. The structural adaptability of LlPR-10.2B and its ability to bind different cytokinins suggest that this protein, and perhaps other PR-10 proteins as well, can act as a reservoir of cytokinin molecules in the aqueous environment of a plant cell.
Cytokinin-induced structural adaptability of a Lupinus luteus PR-10 protein.,Fernandes H, Bujacz A, Bujacz G, Jelen F, Jasinski M, Kachlicki P, Otlewski J, Sikorski MM, Jaskolski M FEBS J. 2009 Mar;276(6):1596-609. Epub 2009 Feb 11. PMID:19220853[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Fernandes H, Pasternak O, Bujacz G, Bujacz A, Sikorski MM, Jaskolski M. Lupinus luteus pathogenesis-related protein as a reservoir for cytokinin. J Mol Biol. 2008 May 16;378(5):1040-51. Epub 2008 Mar 19. PMID:18406424 doi:10.1016/j.jmb.2008.03.027
- ↑ Fernandes H, Bujacz A, Bujacz G, Jelen F, Jasinski M, Kachlicki P, Otlewski J, Sikorski MM, Jaskolski M. Cytokinin-induced structural adaptability of a Lupinus luteus PR-10 protein. FEBS J. 2009 Mar;276(6):1596-609. Epub 2009 Feb 11. PMID:19220853 doi:10.1111/j.1742-4658.2009.06892.x
- ↑ Sliwiak J, Sikorski M, Jaskolski M. PR-10 proteins as potential mediators of melatonin-cytokinin cross-talk in plants: crystallographic studies of LlPR-10.2B isoform from yellow lupine. FEBS J. 2018 Apr 6. doi: 10.1111/febs.14455. PMID:29630775 doi:http://dx.doi.org/10.1111/febs.14455
- ↑ Fernandes H, Bujacz A, Bujacz G, Jelen F, Jasinski M, Kachlicki P, Otlewski J, Sikorski MM, Jaskolski M. Cytokinin-induced structural adaptability of a Lupinus luteus PR-10 protein. FEBS J. 2009 Mar;276(6):1596-609. Epub 2009 Feb 11. PMID:19220853 doi:10.1111/j.1742-4658.2009.06892.x
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