2j5g
From Proteopedia
(New page: 200px<br /> <applet load="2j5g" size="450" color="white" frame="true" align="right" spinBox="true" caption="2j5g, resolution 1.46Å" /> '''THE NATIVE STRUCTUR...) |
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==About this Structure== | ==About this Structure== | ||
| - | 2J5G is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Anabaena_sp. Anabaena sp.]] with SO4 as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.7.1.7 3.7.1.7]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2J5G OCA]]. | + | 2J5G is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Anabaena_sp. Anabaena sp.]] with SO4 as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Beta-diketone_hydrolase Beta-diketone hydrolase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.7.1.7 3.7.1.7]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2J5G OCA]]. |
==Reference== | ==Reference== | ||
Structural characterization of a beta-diketone hydrolase from the cyanobacterium Anabaena sp. PCC 7120 in native and product-bound forms, a coenzyme A-independent member of the crotonase suprafamily., Bennett JP, Whittingham JL, Brzozowski AM, Leonard PM, Grogan G, Biochemistry. 2007 Jan 9;46(1):137-44. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17198383 17198383] | Structural characterization of a beta-diketone hydrolase from the cyanobacterium Anabaena sp. PCC 7120 in native and product-bound forms, a coenzyme A-independent member of the crotonase suprafamily., Bennett JP, Whittingham JL, Brzozowski AM, Leonard PM, Grogan G, Biochemistry. 2007 Jan 9;46(1):137-44. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17198383 17198383] | ||
[[Category: Anabaena sp.]] | [[Category: Anabaena sp.]] | ||
| + | [[Category: Beta-diketone hydrolase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Bennett, J.P.]] | [[Category: Bennett, J.P.]] | ||
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[[Category: lyase]] | [[Category: lyase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:33:36 2007'' |
Revision as of 09:28, 30 October 2007
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THE NATIVE STRUCTURE OF A BETA-DIKETONE HYDROLASE FROM THE CYANOBACTERIUM ANABAENA SP. PCC 7120
Overview
The gene alr4455 from the well-studied cyanobacterium Anabaena sp. PCC, 7120 encodes a crotonase orthologue that displays beta-diketone hydrolase, activity. Anabaena beta-diketone hydrolase (ABDH), in common with, 6-oxocamphor hydrolase (OCH) from Rhodococcus sp. NCIMB 9784, catalyzes, the desymmetrization of bicyclo[2.2.2]octane-2,6-dione to yield, [(S)-3-oxocyclohexyl]acetic acid, a reaction unusual among the crotonase, superfamily as the substrate is not an acyl-CoA thioester. The structure, of ABDH has been determined to a resolution of 1.5 A in both native and, ligand-bound forms. ABDH forms a hexamer similar to OCH and features one, active site per enzyme monomer. The arrangement of side chains in the, active site indicates that while the catalytic chemistry may be conserved, in OCH ... [(full description)]
About this Structure
2J5G is a [Single protein] structure of sequence from [Anabaena sp.] with SO4 as [ligand]. Active as [Beta-diketone hydrolase], with EC number [3.7.1.7]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Structural characterization of a beta-diketone hydrolase from the cyanobacterium Anabaena sp. PCC 7120 in native and product-bound forms, a coenzyme A-independent member of the crotonase suprafamily., Bennett JP, Whittingham JL, Brzozowski AM, Leonard PM, Grogan G, Biochemistry. 2007 Jan 9;46(1):137-44. PMID:17198383
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