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1ggw

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[[Image:1ggw.gif|left|200px]]
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[[Image:1ggw.png|left|200px]]
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{{STRUCTURE_1ggw| PDB=1ggw | SCENE= }}
{{STRUCTURE_1ggw| PDB=1ggw | SCENE= }}
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'''CDC4P FROM SCHIZOSACCHAROMYCES POMBE'''
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===CDC4P FROM SCHIZOSACCHAROMYCES POMBE===
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==Overview==
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The Schizosaccharomyces pombe Cdc4 protein is required for the formation and function of the contractile ring, presumably acting as a myosin light chain. By using NMR spectroscopy, we demonstrate that purified Cdc4p is a monomeric protein with two structurally independent domains, each exhibiting a fold reminiscent of the EF-hand class of calcium-binding proteins. Although Cdc4p has one potentially functional calcium-binding site, it does not bind calcium in vitro. Three variants of Cdc4p containing single point mutations responsible for temperature-sensitive arrest of the cell cycle at cytokinesis (Gly-19 to Glu, Gly-82 to Asp, and Gly-107 to Ser) were also characterized by NMR and circular dichroism spectroscopy. In each case, the amino acid substitution only leads to small perturbations in the conformation of the protein. Furthermore, thermal unfolding studies indicate that, like wild-type Cdc4p, the three mutant forms are all extremely stable, remaining completely folded at temperatures significantly above those causing failure of cytokinesis in intact cells. Therefore, the altered phenotype must arise directly from a disruption of the function of Cdc4p rather than indirectly through a disruption of its overall structure. Several mutant alleles of Cdc4p also show interallelic complementation in diploid cells. This phenomenon can be explained if Cdcp4 has more than one essential function or, alternatively, if two mutant proteins assemble to form a functional complex. Based on the structure of Cdc4p, possible models for interallelic complementation including interactions with partner proteins and the formation of a myosin complex with Cdc4p fulfilling the role of both an essential and regulatory light chain are proposed.
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(as it appears on PubMed at http://www.pubmed.gov), where 11087750 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11087750}}
==About this Structure==
==About this Structure==
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1GGW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GGW OCA].
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1GGW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GGW OCA].
==Reference==
==Reference==
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[[Category: Ef-hand]]
[[Category: Ef-hand]]
[[Category: Light chain]]
[[Category: Light chain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:32:42 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 05:15:58 2008''

Revision as of 02:16, 1 July 2008

Image:1ggw.png

Template:STRUCTURE 1ggw

CDC4P FROM SCHIZOSACCHAROMYCES POMBE

Template:ABSTRACT PUBMED 11087750

About this Structure

1GGW is a Single protein structure of sequence from Schizosaccharomyces pombe. Full experimental information is available from OCA.

Reference

Structure of Cdc4p, a contractile ring protein essential for cytokinesis in Schizosaccharomyces pombe., Slupsky CM, Desautels M, Huebert T, Zhao R, Hemmingsen SM, McIntosh LP, J Biol Chem. 2001 Feb 23;276(8):5943-51. Epub 2000 Nov 21. PMID:11087750

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