Journal:Acta Cryst D:S2059798322002157
From Proteopedia
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<StructureSection load='' size='450' side='right' scene='underdevelopment' caption=''> | <StructureSection load='' size='450' side='right' scene='underdevelopment' caption=''> | ||
| - | ===The X-ray crystallography phase problem solved thanks to AlphaFold and RoseTTAFold models : a case study report | + | ===The X-ray crystallography phase problem solved thanks to AlphaFold and RoseTTAFold models: a case study report=== |
<big>Irène Barbarin-Bocahu1, Marc Graille</big> <ref>doi: 10.1107/S2059798322002157</ref> | <big>Irène Barbarin-Bocahu1, Marc Graille</big> <ref>doi: 10.1107/S2059798322002157</ref> | ||
<hr/> | <hr/> | ||
<b>Molecular Tour</b><br> | <b>Molecular Tour</b><br> | ||
| + | Depending on their amino acid sequences, the proteins fold into specific three-dimensional (3D) structures, which are crucial to fulfill their cellular and biochemical functions. The knowledge of the 3D structure of all the proteins is then very important to appreciate their biological and biochemical functions as well as the potential impact of mutations associated with diseases. Very high accuracy in protein 3D structure prediction has recently been reached by the deep-learning based programs AlphaFold or RoseTTAFold. | ||
| + | Here, we take advantage of these high quality models to solve the crystal structure of a yeast protein involved in an mRNA quality control pathway. This article illustrates one of the important applications of 3D protein structure models generated by AlphaFold or RoseTTAFold programs, i.e. in solving the X-ray crystallography phase problem. We discuss the different strategies to generate search models. Finally, this article also contributes to the validation of the very high quality of these models. | ||
<b>References</b><br> | <b>References</b><br> | ||
Revision as of 13:07, 24 February 2022
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This page complements a publication in scientific journals and is one of the Proteopedia's Interactive 3D Complement pages. For aditional details please see I3DC.
