2p6r
From Proteopedia
(Difference between revisions)
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<StructureSection load='2p6r' size='340' side='right'caption='[[2p6r]], [[Resolution|resolution]] 3.00Å' scene=''> | <StructureSection load='2p6r' size='340' side='right'caption='[[2p6r]], [[Resolution|resolution]] 3.00Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[2p6r]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/ | + | <table><tr><td colspan='2'>[[2p6r]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P6R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2P6R FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2p6r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p6r OCA], [https://pdbe.org/2p6r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2p6r RCSB], [https://www.ebi.ac.uk/pdbsum/2p6r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2p6r ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2p6r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p6r OCA], [https://pdbe.org/2p6r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2p6r RCSB], [https://www.ebi.ac.uk/pdbsum/2p6r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2p6r ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2p6r ConSurf]. | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2p6r ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | To reveal the mechanism of processive strand separation by superfamily-2 (SF2) 3'-->5' helicases, we determined apo and DNA-bound crystal structures of archaeal Hel308, a helicase that unwinds lagging strands and is related to human DNA polymerase theta. Our structure captures the duplex-unwinding reaction, shows that initial strand separation does not require ATP and identifies a prominent beta-hairpin loop as the unwinding element. Similar loops in hepatitis C virus NS3 helicase and RNA-decay factors support the idea that this duplex-unwinding mechanism is applicable to a broad subset of SF2 helicases. Comparison with ATP-bound SF2 enzymes suggests that ATP promotes processive unwinding of 1 base pair by ratchet-like transport of the 3' product strand. Our results provide a first structural framework for strand separation by processive SF2 3'-->5' helicases and reveal important mechanistic differences from SF1 helicases. | ||
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| - | Structural basis for DNA duplex separation by a superfamily-2 helicase.,Buttner K, Nehring S, Hopfner KP Nat Struct Mol Biol. 2007 Jul;14(7):647-52. Epub 2007 Jun 10. PMID:17558417<ref>PMID:17558417</ref> | ||
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 2p6r" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
*[[Helicase 3D structures|Helicase 3D structures]] | *[[Helicase 3D structures|Helicase 3D structures]] | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Archaeoglobus fulgidus]] |
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Buettner | + | [[Category: Buettner K]] |
| - | [[Category: Hopfner | + | [[Category: Hopfner KP]] |
| - | [[Category: Nehring | + | [[Category: Nehring S]] |
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Current revision
Crystal structure of superfamily 2 helicase Hel308 in complex with unwound DNA
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