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1gjj

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[[Image:1gjj.gif|left|200px]]
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[[Image:1gjj.png|left|200px]]
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{{STRUCTURE_1gjj| PDB=1gjj | SCENE= }}
{{STRUCTURE_1gjj| PDB=1gjj | SCENE= }}
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'''N-TERMINAL CONSTANT REGION OF THE NUCLEAR ENVELOPE PROTEIN LAP2'''
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===N-TERMINAL CONSTANT REGION OF THE NUCLEAR ENVELOPE PROTEIN LAP2===
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==Overview==
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The nuclear envelope proteins LAP2, emerin and MAN1 share a conserved approximately 40-residue 'LEM' motif. Loss of emerin causes Emery-Dreifuss muscular dystrophy. We have solved the solution NMR structure of the constant region of human LAP2 (residues 1-168). Human LAP2(1-168) has two structurally independent, non-interacting domains located at residues 1-50 ('LAP2-N') and residues 111-152 (LEM-domain), connected by an approximately 60-residue flexible linker. The two domains are structurally homologous, comprising a helical turn followed by two helices connected by an 11-12-residue loop. This motif is shared by subdomains of T4 endonuclease VII and transcription factor rho, despite negligible (&lt; or =15%) sequence identity. NMR chemical shift mapping demonstrated that the LEM-domain binds BAF (barrier-to-autointegration factor), whereas LAP2-N binds DNA. Both binding surfaces comprise helix 1, the N-terminus of helix 2 and the inter-helical loop. Binding selectivity is determined by the nature of the surface residues in these binding sites, which are predominantly positively charged for LAP2-N and hydrophobic for the LEM-domain. Thus, LEM and LEM-like motifs form a common structure that evolution has customized for binding to BAF or DNA.
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(as it appears on PubMed at http://www.pubmed.gov), where 11500367 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11500367}}
==About this Structure==
==About this Structure==
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1GJJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GJJ OCA].
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1GJJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GJJ OCA].
==Reference==
==Reference==
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[[Category: Lem domain]]
[[Category: Lem domain]]
[[Category: Multidimensional nmr dipolar coupling]]
[[Category: Multidimensional nmr dipolar coupling]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:39:19 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 05:23:40 2008''

Revision as of 02:23, 1 July 2008

Image:1gjj.png

Template:STRUCTURE 1gjj

N-TERMINAL CONSTANT REGION OF THE NUCLEAR ENVELOPE PROTEIN LAP2

Template:ABSTRACT PUBMED 11500367

About this Structure

1GJJ is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

Solution structure of the constant region of nuclear envelope protein LAP2 reveals two LEM-domain structures: one binds BAF and the other binds DNA., Cai M, Huang Y, Ghirlando R, Wilson KL, Craigie R, Clore GM, EMBO J. 2001 Aug 15;20(16):4399-407. PMID:11500367

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