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1gjw

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{{STRUCTURE_1gjw| PDB=1gjw | SCENE= }}
{{STRUCTURE_1gjw| PDB=1gjw | SCENE= }}
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'''THERMOTOGA MARITIMA MALTOSYLTRANSFERASE COMPLEX WITH MALTOSE'''
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===THERMOTOGA MARITIMA MALTOSYLTRANSFERASE COMPLEX WITH MALTOSE===
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==Overview==
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Maltosyltransferase (MTase) from the hyperthermophile Thermotoga maritima represents a novel maltodextrin glycosyltransferase acting on starch and malto-oligosaccharides. It catalyzes the transfer of maltosyl units from alpha-1,4-linked glucans or malto-oligosaccharides to other alpha-1,4-linked glucans, malto-oligosaccharides or glucose. It belongs to the glycoside hydrolase family 13, which represents a large group of (beta/alpha)(8) barrel proteins sharing a similar active site structure. The crystal structures of MTase and its complex with maltose have been determined at 2.4 A and 2.1 A resolution, respectively. MTase is a homodimer, each subunit of which consists of four domains, two of which are structurally homologous to those of other family 13 enzymes. The catalytic core domain has the (beta/alpha)(8) barrel fold with the active-site cleft formed at the C-terminal end of the barrel. Substrate binding experiments have led to the location of two distinct maltose-binding sites; one lies in the active-site cleft, covering subsites -2 and -1; the other is located in a pocket adjacent to the active-site cleft. The structure of MTase, together with the conservation of active-site residues among family 13 glycoside hydrolases, are consistent with a common double-displacement catalytic mechanism for this enzyme. Analysis of maltose binding in the active site reveals that the transfer of dextrinyl residues longer than a maltosyl unit is prevented by termination of the active-site cleft after the -2 subsite by the side-chain of Lys151 and the stretch of residues 314-317, providing an explanation for the strict transfer specificity of MTase.
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(as it appears on PubMed at http://www.pubmed.gov), where 11545590 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11545590}}
==About this Structure==
==About this Structure==
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[[Category: Maltosyltransferase]]
[[Category: Maltosyltransferase]]
[[Category: Transferase]]
[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 05:24:36 2008''

Revision as of 02:24, 1 July 2008

Image:1gjw.png

Template:STRUCTURE 1gjw

THERMOTOGA MARITIMA MALTOSYLTRANSFERASE COMPLEX WITH MALTOSE

Template:ABSTRACT PUBMED 11545590

About this Structure

1GJW is a Single protein structure of sequence from Thermotoga maritima. Full crystallographic information is available from OCA.

Reference

The crystal structure of Thermotoga maritima maltosyltransferase and its implications for the molecular basis of the novel transfer specificity., Roujeinikova A, Raasch C, Burke J, Baker PJ, Liebl W, Rice DW, J Mol Biol. 2001 Sep 7;312(1):119-31. PMID:11545590

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