2ayj

From Proteopedia

(Difference between revisions)

Current revision

Solution structure of 50S ribosomal protein L40e from Sulfolobus solfataricus

Structural highlights

2ayj is a 1 chain structure with sequence from Saccharolobus solfataricus. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT, TOPSAN

Function

RL40_SACS2

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The ribosomal protein L40E from archaeon Sulfolobus solfataricus is a component of the 50S ribosomal subunit. L40E is a 56-residue, highly basic protein that contains a C4 zinc finger motif, CRKC_X(10)_CRRC. Homologs are found in both archaea and eukaryotes but are not present in bacteria. Eukaryotic genomes encode L40E as a ubiquitin-fusion protein. L40E was absent from the crystal structure of euryarchaeota 50S ribosomal subunit. Here we report the three-dimensional solution structure of L40E by NMR spectroscopy. The structure of L40E is a three-stranded beta-sheet with a simple beta2beta1beta3 topology. There are two unique characteristics revealed by the structure. First, a large and ordered beta2-beta3 loop twists to pack across the one side of the protein. L40E contains a buried polar cluster comprising Lys19, Lys20, Cys22, Asn29, and Cys36. Second, the surface of L40E is almost entirely positively charged. Ten conserved basic residues are positioned on the two sides of the surface. It is likely that binding of zinc is essential in stabilizing the tertiary structure of L40E to act as a scaffold to create a broad positively charged surface for RNA and/or protein recognition.

Solution structure of ribosomal protein L40E, a unique C4 zinc finger protein encoded by archaeon Sulfolobus solfataricus.,Wu B, Lukin J, Yee A, Lemak A, Semesi A, Ramelot TA, Kennedy MA, Arrowsmith CH Protein Sci. 2008 Mar;17(3):589-96. Epub 2008 Jan 24. PMID:18218710^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Wu B, Lukin J, Yee A, Lemak A, Semesi A, Ramelot TA, Kennedy MA, Arrowsmith CH. Solution structure of ribosomal protein L40E, a unique C4 zinc finger protein encoded by archaeon Sulfolobus solfataricus. Protein Sci. 2008 Mar;17(3):589-96. Epub 2008 Jan 24. PMID:18218710 doi:http://dx.doi.org/10.1110/ps.073273008

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ayj"

@@ Line 1: / Line 1: @@
 ==Solution structure of 50S ribosomal protein L40e from Sulfolobus solfataricus==
-<StructureSection load='2ayj' size='340' side='right'caption='[[2ayj]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='2ayj' size='340' side='right'caption='[[2ayj]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2ayj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_35091 Atcc 35091]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AYJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AYJ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2ayj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus Saccharolobus solfataricus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AYJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AYJ FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rpl40e ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2287 ATCC 35091])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ayj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ayj OCA], [https://pdbe.org/2ayj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ayj RCSB], [https://www.ebi.ac.uk/pdbsum/2ayj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ayj ProSAT], [https://www.topsan.org/Proteins/NESGC/2ayj TOPSAN]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/RL40_SACS2 RL40_SACS2]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 31: / Line 33: @@
 __TOC__
 </StructureSection>
-[[Category: Atcc 35091]]
 [[Category: Large Structures]]
-[[Category: Arrowsmith, C H]]
+[[Category: Saccharolobus solfataricus]]
-[[Category: Edward, A]]
+[[Category: Arrowsmith CH]]
-[[Category: Kennedy, M]]
+[[Category: Edward A]]
-[[Category: Lemak, A]]
+[[Category: Kennedy M]]
-[[Category: Lukin, J]]
+[[Category: Lemak A]]
-[[Category: Structural genomic]]
+[[Category: Lukin J]]
-[[Category: Ramelot, T]]
+[[Category: Ramelot T]]
-[[Category: Semesi, A]]
+[[Category: Semesi A]]
-[[Category: Wu, B]]
+[[Category: Wu B]]
-[[Category: Yee, A]]
+[[Category: Yee A]]
-[[Category: Beta-strand protein]]
-[[Category: Nesg]]
-[[Category: PSI, Protein structure initiative]]
-[[Category: Translation]]
-[[Category: Zn-binding]]

2ayj

From Proteopedia

Current revision

Solution structure of 50S ribosomal protein L40e from Sulfolobus solfataricus

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox