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7k0t
From Proteopedia
(Difference between revisions)
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==Cryo-EM structure of rabbit RyR1 in the presence of AMP-PCP in nanodisc== | ==Cryo-EM structure of rabbit RyR1 in the presence of AMP-PCP in nanodisc== | ||
| - | <StructureSection load='7k0t' size='340' side='right'caption='[[7k0t]]' scene=''> | + | <StructureSection load='7k0t' size='340' side='right'caption='[[7k0t]], [[Resolution|resolution]] 4.30Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7K0T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7K0T FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7k0t]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7K0T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7K0T FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7k0t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7k0t OCA], [https://pdbe.org/7k0t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7k0t RCSB], [https://www.ebi.ac.uk/pdbsum/7k0t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7k0t ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC+ACID+ADENYLATE+ESTER'>ACP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[7tdg|7tdg]]</div></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7k0t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7k0t OCA], [https://pdbe.org/7k0t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7k0t RCSB], [https://www.ebi.ac.uk/pdbsum/7k0t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7k0t ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Activation of the intracellular Ca(2+) channel ryanodine receptor (RyR) triggers a cytosolic Ca(2+) surge, while elevated cytosolic Ca(2+) inhibits the channel in a negative feedback mechanism. Cryo-EM of rabbit RyR1 embedded in nanodiscs under partially inactivating Ca(2+) conditions revealed an open and a closed-inactivated conformation. Ca(2+) binding to the high affinity site engages the central and C-terminal domains into a block, which pries the S6 four-helix bundle open. Further rotation of this block pushes S6 toward the central axis, closing (inactivating) the channel. Main characteristics of the Ca(2+)-inactivated conformation are downward conformation of the cytoplasmic assembly and tightly-knit subunit interface contributed by a fully occupied Ca(2+) activation site, two inter-subunit resolved lipids, and two salt bridges between the EF hand domain and the S2-S3 loop validated by disease-causing mutations. The structural insight illustrates the prior Ca(2+) activation prerequisite for Ca(2+) inactivation and provides for seamless transition from inactivated to closed conformations. | ||
| + | |||
| + | Ca(2+)-inactivation of the mammalian ryanodine receptor type 1 in a lipidic environment revealed by cryo-EM.,Nayak AR, Samso M Elife. 2022 Mar 8;11. pii: 75568. doi: 10.7554/eLife.75568. PMID:35257661<ref>PMID:35257661</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7k0t" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Ryanodine receptor 3D structures|Ryanodine receptor 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Nayak | + | [[Category: Oryctolagus cuniculus]] |
| - | [[Category: Samso M]] | + | [[Category: Nayak, A R]] |
| + | [[Category: Samso, M]] | ||
| + | [[Category: Atp]] | ||
| + | [[Category: Excitation-contraction coupling]] | ||
| + | [[Category: Intracellular calcium channel]] | ||
| + | [[Category: Ryanodine receptor]] | ||
| + | [[Category: Ryr1]] | ||
| + | [[Category: Transport protein]] | ||
Revision as of 07:16, 16 March 2022
Cryo-EM structure of rabbit RyR1 in the presence of AMP-PCP in nanodisc
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