1e03
From Proteopedia
(New page: 200px<br /> <applet load="1e03" size="450" color="white" frame="true" align="right" spinBox="true" caption="1e03, resolution 2.9Å" /> '''PLASMA ALPHA ANTITHR...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1e03.gif|left|200px]]<br /> | + | [[Image:1e03.gif|left|200px]]<br /><applet load="1e03" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1e03" size=" | + | |
caption="1e03, resolution 2.9Å" /> | caption="1e03, resolution 2.9Å" /> | ||
'''PLASMA ALPHA ANTITHROMBIN-III AND PENTASACCHARIDE'''<br /> | '''PLASMA ALPHA ANTITHROMBIN-III AND PENTASACCHARIDE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Antithrombin, a plasma serpin, is relatively inactive as an inhibitor of | + | Antithrombin, a plasma serpin, is relatively inactive as an inhibitor of the coagulation proteases until it binds to the heparan side chains that line the microvasculature. The binding specifically occurs to a core pentasaccharide present both in the heparans and in their therapeutic derivative heparin. The accompanying conformational change of antithrombin is revealed in a 2.9-A structure of a dimer of latent and active antithrombins, each in complex with the high-affinity pentasaccharide. Inhibitory activation results from a shift in the main sheet of the molecule from a partially six-stranded to a five-stranded form, with extrusion of the reactive center loop to give a more exposed orientation. There is a tilting and elongation of helix D with the formation of a 2-turn helix P between the C and D helices. Concomitant conformational changes at the heparin binding site explain both the initial tight binding of antithrombin to the heparans and the subsequent release of the antithrombin-protease complex into the circulation. The pentasaccharide binds by hydrogen bonding of its sulfates and carboxylates to Arg-129 and Lys-125 in the D-helix, to Arg-46 and Arg-47 in the A-helix, to Lys-114 and Glu-113 in the P-helix, and to Lys-11 and Arg-13 in a cleft formed by the amino terminus. This clear definition of the binding site will provide a structural basis for developing heparin analogues that are more specific toward their intended target antithrombin and therefore less likely to exhibit side effects. |
==About this Structure== | ==About this Structure== | ||
| - | 1E03 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with NAG and NTP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1E03 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=NAG:'>NAG</scene> and <scene name='pdbligand=NTP:'>NTP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E03 OCA]. |
==Reference== | ==Reference== | ||
| Line 14: | Line 13: | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Abrahams, J | + | [[Category: Abrahams, J P.]] |
| - | [[Category: Carrell, R | + | [[Category: Carrell, R W.]] |
[[Category: Jin, L.]] | [[Category: Jin, L.]] | ||
| - | [[Category: Mccoy, A | + | [[Category: Mccoy, A J.]] |
[[Category: Skinner, R.]] | [[Category: Skinner, R.]] | ||
[[Category: NAG]] | [[Category: NAG]] | ||
| Line 23: | Line 22: | ||
[[Category: serpin]] | [[Category: serpin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:22:19 2008'' |
Revision as of 10:22, 21 February 2008
|
PLASMA ALPHA ANTITHROMBIN-III AND PENTASACCHARIDE
Overview
Antithrombin, a plasma serpin, is relatively inactive as an inhibitor of the coagulation proteases until it binds to the heparan side chains that line the microvasculature. The binding specifically occurs to a core pentasaccharide present both in the heparans and in their therapeutic derivative heparin. The accompanying conformational change of antithrombin is revealed in a 2.9-A structure of a dimer of latent and active antithrombins, each in complex with the high-affinity pentasaccharide. Inhibitory activation results from a shift in the main sheet of the molecule from a partially six-stranded to a five-stranded form, with extrusion of the reactive center loop to give a more exposed orientation. There is a tilting and elongation of helix D with the formation of a 2-turn helix P between the C and D helices. Concomitant conformational changes at the heparin binding site explain both the initial tight binding of antithrombin to the heparans and the subsequent release of the antithrombin-protease complex into the circulation. The pentasaccharide binds by hydrogen bonding of its sulfates and carboxylates to Arg-129 and Lys-125 in the D-helix, to Arg-46 and Arg-47 in the A-helix, to Lys-114 and Glu-113 in the P-helix, and to Lys-11 and Arg-13 in a cleft formed by the amino terminus. This clear definition of the binding site will provide a structural basis for developing heparin analogues that are more specific toward their intended target antithrombin and therefore less likely to exhibit side effects.
About this Structure
1E03 is a Single protein structure of sequence from Homo sapiens with and as ligands. Full crystallographic information is available from OCA.
Reference
The anticoagulant activation of antithrombin by heparin., Jin L, Abrahams JP, Skinner R, Petitou M, Pike RN, Carrell RW, Proc Natl Acad Sci U S A. 1997 Dec 23;94(26):14683-8. PMID:9405673
Page seeded by OCA on Thu Feb 21 12:22:19 2008
Categories: Homo sapiens | Single protein | Abrahams, J P. | Carrell, R W. | Jin, L. | Mccoy, A J. | Skinner, R. | NAG | NTP | Serpin
