1gmm

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{{STRUCTURE_1gmm| PDB=1gmm | SCENE= }}
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'''CARBOHYDRATE BINDING MODULE CBM6 FROM XYLANASE U CLOSTRIDIUM THERMOCELLUM'''
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===CARBOHYDRATE BINDING MODULE CBM6 FROM XYLANASE U CLOSTRIDIUM THERMOCELLUM===
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==Overview==
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Polysaccharide-degrading enzymes are generally modular proteins that contain non-catalytic carbohydrate-binding modules (CBMs), which potentiate the activity of the catalytic module. CBMs have been grouped into sequence-based families, and three-dimensional structural data are available for half of these families. Clostridium thermocellum xylanase 11A is a modular enzyme that contains a CBM from family 6 (CBM6), for which no structural data are available. We have determined the crystal structure of this module to a resolution of 2.1 A. The protein is a beta-sandwich that contains two potential ligand-binding clefts designated cleft A and B. The CBM interacts primarily with xylan, and NMR spectroscopy coupled with site-directed mutagenesis identified cleft A, containing Trp-92, Tyr-34, and Asn-120, as the ligand-binding site. The overall fold of CBM6 is similar to proteins in CBM families 4 and 22, although surprisingly the ligand-binding site in CBM4 and CBM22 is equivalent to cleft B in CBM6. These structural data define a superfamily of CBMs, comprising CBM4, CBM6, and CBM22, and demonstrate that, although CBMs have evolved from a relatively small number of ancestors, the structural elements involved in ligand recognition have been assembled at different locations on the ancestral scaffold.
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==About this Structure==
==About this Structure==
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[[Category: Cbm family 6]]
[[Category: Cbm family 6]]
[[Category: Xylan binding]]
[[Category: Xylan binding]]
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Revision as of 02:31, 1 July 2008

Image:1gmm.png

Template:STRUCTURE 1gmm

CARBOHYDRATE BINDING MODULE CBM6 FROM XYLANASE U CLOSTRIDIUM THERMOCELLUM

Template:ABSTRACT PUBMED 11673472

About this Structure

1GMM is a Single protein structure of sequence from Clostridium thermocellum. Full crystallographic information is available from OCA.

Reference

The location of the ligand-binding site of carbohydrate-binding modules that have evolved from a common sequence is not conserved., Czjzek M, Bolam DN, Mosbah A, Allouch J, Fontes CM, Ferreira LM, Bornet O, Zamboni V, Darbon H, Smith NL, Black GW, Henrissat B, Gilbert HJ, J Biol Chem. 2001 Dec 21;276(51):48580-7. Epub 2001 Oct 22. PMID:11673472

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