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1gmv

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[[Image:1gmv.gif|left|200px]]
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{{STRUCTURE_1gmv| PDB=1gmv | SCENE= }}
{{STRUCTURE_1gmv| PDB=1gmv | SCENE= }}
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'''STRUCTURE OF UREE'''
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===STRUCTURE OF UREE===
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==Overview==
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UreE is proposed to be a metallochaperone that delivers nickel ions to urease during activation of this bacterial virulence factor. Wild-type Klebsiella aerogenes UreE binds approximately six nickel ions per homodimer, whereas H144*UreE (a functional C-terminal truncated variant) was previously reported to bind two. We determined the structure of H144*UreE by multi-wavelength anomalous diffraction and refined it to 1.5 A resolution. The present structure reveals an Hsp40-like peptide-binding domain, an Atx1-like metal-binding domain, and a flexible C terminus. Three metal-binding sites per dimer, defined by structural analysis of Cu-H144*UreE, are on the opposite face of the Atx1-like domain than observed in the copper metallochaperone. One metal bridges the two subunits via the pair of His-96 residues, whereas the other two sites involve metal coordination by His-110 and His-112 within each subunit. In contrast to the copper metallochaperone mechanism involving thiol ligand exchanges between structurally similar chaperones and target proteins, we propose that the Hsp40-like module interacts with urease apoprotein and/or other urease accessory proteins, while the Atx1-like domain delivers histidyl-bound nickel to the urease active site.
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The line below this paragraph, {{ABSTRACT_PUBMED_11591723}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 11591723 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11591723}}
==About this Structure==
==About this Structure==
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[[Category: Song, H K.]]
[[Category: Song, H K.]]
[[Category: Metallochaperone]]
[[Category: Metallochaperone]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:45:44 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 05:31:52 2008''

Revision as of 02:31, 1 July 2008

Image:1gmv.png

Template:STRUCTURE 1gmv

STRUCTURE OF UREE

Template:ABSTRACT PUBMED 11591723

About this Structure

1GMV is a Single protein structure of sequence from Klebsiella aerogenes. Full crystallographic information is available from OCA.

Reference

Crystal structure of Klebsiella aerogenes UreE, a nickel-binding metallochaperone for urease activation., Song HK, Mulrooney SB, Huber R, Hausinger RP, J Biol Chem. 2001 Dec 28;276(52):49359-64. Epub 2001 Oct 8. PMID:11591723

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