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3gx8
From Proteopedia
(Difference between revisions)
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<StructureSection load='3gx8' size='340' side='right'caption='[[3gx8]], [[Resolution|resolution]] 1.67Å' scene=''> | <StructureSection load='3gx8' size='340' side='right'caption='[[3gx8]], [[Resolution|resolution]] 1.67Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[3gx8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/ | + | <table><tr><td colspan='2'>[[3gx8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GX8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GX8 FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.673Å</td></tr> |
| - | <tr id=' | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gx8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gx8 OCA], [https://pdbe.org/3gx8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gx8 RCSB], [https://www.ebi.ac.uk/pdbsum/3gx8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gx8 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gx8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gx8 OCA], [https://pdbe.org/3gx8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gx8 RCSB], [https://www.ebi.ac.uk/pdbsum/3gx8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gx8 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
| - | + | [https://www.uniprot.org/uniprot/GLRX5_YEAST GLRX5_YEAST] Monothiol glutaredoxin involved in iron-sulfur biogenesis. Required for normal iron homeostasis. Protects cells against oxidative damage due to reactive oxygen species.<ref>PMID:11950925</ref> | |
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3gx8 ConSurf]. | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3gx8 ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | Grx5 from the yeast Saccharomyces cerevisiae is a monothiol glutaredoxin that is involved in iron-sulfur cluster biogenesis. Here, yeast Grx5 was cloned and overproduced in Escherichia coli. The purified protein was crystallized using the hanging-drop vapour-diffusion method. Diffraction data for Grx5 were collected to 1.67 A resolution. The crystal of Grx5 belonged to space group R3, with unit-cell parameters a = b = 85.12, c = 48.95 A, alpha = beta = 90.00, gamma = 120.00 degrees . | ||
| - | |||
| - | Cloning, overproduction, purification, crystallization and preliminary X-ray diffraction analysis of yeast glutaredoxin Grx5.,Wang Y, He YX, Yu J, Zhou CZ Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Jun 1;65(Pt, 6):651-3. Epub 2009 May 23. PMID:19478456<ref>PMID:19478456</ref> | ||
| - | |||
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 3gx8" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: Atcc 18824]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Chen | + | [[Category: Saccharomyces cerevisiae]] |
| - | [[Category: He | + | [[Category: Chen Y]] |
| - | [[Category: Wang | + | [[Category: He YX]] |
| - | [[Category: Xiong | + | [[Category: Wang Y]] |
| - | [[Category: Yu | + | [[Category: Xiong Y]] |
| - | [[Category: Zhou | + | [[Category: Yu J]] |
| - | + | [[Category: Zhou CZ]] | |
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Current revision
Structural and biochemical characterization of yeast monothiol glutaredoxin Grx5
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Categories: Large Structures | Saccharomyces cerevisiae | Chen Y | He YX | Wang Y | Xiong Y | Yu J | Zhou CZ
