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1go8

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{{STRUCTURE_1go8| PDB=1go8 | SCENE= }}
{{STRUCTURE_1go8| PDB=1go8 | SCENE= }}
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'''THE METZINCIN'S METHIONINE: PRTC M226L MUTANT'''
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===THE METZINCIN'S METHIONINE: PRTC M226L MUTANT===
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==Overview==
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Serralysins are a family of metalloproteases secreted by Gram-negative bacteria into the medium in the form of inactive zymogens. Usually, all serralysin secretors have on the same operon a gene coding for a periplasmic 10-kDa protein, which is an inhibitor of the secreted protease. The recent characterization of the inhibitor of the alkaline protease from Pseudomonas aeruginosa revealed a surprisingly low dissociation constant of 4 pm, contrary to earlier studies on homologous systems, where inhibition constants in the microm range were reported. To approach a more accurate understanding, the crystal structure of the complex between inhibitor and protease from P. aeruginosa was determined at 1.74 A resolution and refined to R(free) = 0.204. The structure reported here shows clearly that the N terminus of the inhibitor forms a coordinative bond to the catalytic Zn(2+) ion with a nitrogen-zinc distance of 2.17 A. We conclude that this interaction adds substantially to the complex stability and show also that similar interactions are found in other metzincin-inhibitor complexes.
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(as it appears on PubMed at http://www.pubmed.gov), where 11445573 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11445573}}
==About this Structure==
==About this Structure==
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[[Category: Metalloprotease]]
[[Category: Metalloprotease]]
[[Category: Protease]]
[[Category: Protease]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:48:54 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 05:35:49 2008''

Revision as of 02:35, 1 July 2008

Image:1go8.png

Template:STRUCTURE 1go8

THE METZINCIN'S METHIONINE: PRTC M226L MUTANT

Template:ABSTRACT PUBMED 11445573

About this Structure

1GO8 is a Single protein structure of sequence from Erwinia chrysanthemi. Full crystallographic information is available from OCA.

Reference

Crystal structure of a complex between Pseudomonas aeruginosa alkaline protease and its cognate inhibitor: inhibition by a zinc-NH2 coordinative bond., Hege T, Feltzer RE, Gray RD, Baumann U, J Biol Chem. 2001 Sep 14;276(37):35087-92. Epub 2001 Jul 9. PMID:11445573

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