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1gof

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{{STRUCTURE_1gof| PDB=1gof | SCENE= }}
{{STRUCTURE_1gof| PDB=1gof | SCENE= }}
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'''NOVEL THIOETHER BOND REVEALED BY A 1.7 ANGSTROMS CRYSTAL STRUCTURE OF GALACTOSE OXIDASE'''
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===NOVEL THIOETHER BOND REVEALED BY A 1.7 ANGSTROMS CRYSTAL STRUCTURE OF GALACTOSE OXIDASE===
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==Overview==
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Galactose oxidase is an extracellular enzyme secreted by the fungus Dactylium dendroides. It is monomeric, with a relative molecular mass of 68,000, catalyses the stereospecific oxidation of a broad range of primary alcohol substrates and possesses a unique mononuclear copper site essential for catalysing a two-electron transfer reaction during the oxidation of primary alcohols to corresponding aldehydes. Recent evidence arguing against a Cu(III)-Cu(I) couple implies the existence of a second redox-active site proposed to involve pyrroloquinoline quinone or a tyrosine radical. We now report the crystal structure of galactose oxidase at 1.7 A resolution. This reveals a unique structural feature at the copper site with a novel thioether bond linking Cys 228 and Tyr 272 in a stacking interaction with Trp 290. We propose that these molecular components stabilize the protein free-radical species essential for catalysis and thus provide a 'built-in' secondary cofactor. This feature may represent a new mechanism for mediating electron transfer in metalloenzymes in the absence of exogenous cofactors.
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{{ABSTRACT_PUBMED_2002850}}
==About this Structure==
==About this Structure==
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[[Category: Knowles, P F.]]
[[Category: Knowles, P F.]]
[[Category: Phillips, S E.V.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:49:18 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 05:36:11 2008''

Revision as of 02:36, 1 July 2008

Image:1gof.png

Template:STRUCTURE 1gof

NOVEL THIOETHER BOND REVEALED BY A 1.7 ANGSTROMS CRYSTAL STRUCTURE OF GALACTOSE OXIDASE

Template:ABSTRACT PUBMED 2002850

About this Structure

1GOF is a Single protein structure of sequence from Hypomyces rosellus. Full crystallographic information is available from OCA.

Reference

Novel thioether bond revealed by a 1.7 A crystal structure of galactose oxidase., Ito N, Phillips SE, Stevens C, Ogel ZB, McPherson MJ, Keen JN, Yadav KD, Knowles PF, Nature. 1991 Mar 7;350(6313):87-90. PMID:2002850

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