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2uwm

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C-TERMINAL DOMAIN(WH2-WH4) OF ELONGATION FACTOR SELB IN COMPLEX WITH SECIS RNA

Structural highlights

2uwm is a 4 chain structure with sequence from Moorella thermoacetica. The August 2008 RCSB PDB Molecule of the Month feature on Selenocysteine Synthase by David Goodsell is 10.2210/rcsb_pdb/mom_2008_8. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.31Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q2RFK4_MOOTA

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Selenocysteine (Sec) is the "21st" amino acid and is genetically encoded by an unusual incorporation system. The stop codon UGA becomes a Sec codon when the selenocysteine insertion sequence (SECIS) exists downstream of UGA. Sec incorporation requires a specific elongation factor, SelB, which recognizes tRNA(Sec) via use of an EF-Tu-like domain and the SECIS mRNA hairpin via use of a C-terminal domain (SelB-C). SelB functions in multiple translational steps: binding to SECIS mRNA and tRNA(Sec), delivery of tRNA(Sec) onto an A site, GTP hydrolysis, and release from tRNA and mRNA. However, this dynamic mechanism remains to be revealed. Here, we report a large domain rearrangement in the structure of SelB-C complexed with RNA. Surprisingly, the interdomain region forms new interactions with the phosphate backbone of a neighboring RNA, distinct from SECIS RNA binding. This SelB-RNA interaction is sequence independent, possibly reflecting SelB-tRNA/-rRNA recognitions. Based on these data, the dynamic SelB-ribosome-mRNA-tRNA interactions will be discussed.

Structural basis for dynamic interdomain movement and RNA recognition of the selenocysteine-specific elongation factor SelB.,Ose T, Soler N, Rasubala L, Kuroki K, Kohda D, Fourmy D, Yoshizawa S, Maenaka K Structure. 2007 May;15(5):577-86. PMID:17502103^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ose T, Soler N, Rasubala L, Kuroki K, Kohda D, Fourmy D, Yoshizawa S, Maenaka K. Structural basis for dynamic interdomain movement and RNA recognition of the selenocysteine-specific elongation factor SelB. Structure. 2007 May;15(5):577-86. PMID:17502103 doi:10.1016/j.str.2007.03.007

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2uwm"

@@ Line 3: / Line 3: @@
 <StructureSection load='2uwm' size='340' side='right'caption='[[2uwm]], [[Resolution|resolution]] 2.31&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2uwm]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_35608 Atcc 35608]. The August 2008 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Selenocysteine Synthase''  by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2008_8 10.2210/rcsb_pdb/mom_2008_8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UWM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2UWM FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2uwm]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Moorella_thermoacetica Moorella thermoacetica]. The August 2008 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Selenocysteine Synthase''  by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2008_8 10.2210/rcsb_pdb/mom_2008_8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UWM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2UWM FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2uwm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uwm OCA], [https://pdbe.org/2uwm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2uwm RCSB], [https://www.ebi.ac.uk/pdbsum/2uwm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2uwm ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.31&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2uwm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uwm OCA], [https://pdbe.org/2uwm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2uwm RCSB], [https://www.ebi.ac.uk/pdbsum/2uwm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2uwm ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q2RFK4_MOOTA Q2RFK4_MOOTA]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 33: / Line 36: @@
 __TOC__
 </StructureSection>
-[[Category: Atcc 35608]]
 [[Category: Large Structures]]
+[[Category: Moorella thermoacetica]]
 [[Category: RCSB PDB Molecule of the Month]]
 [[Category: Selenocysteine Synthase]]
-[[Category: Fourmy, D]]
+[[Category: Fourmy D]]
-[[Category: Kohda, D]]
+[[Category: Kohda D]]
-[[Category: Kuroki, K]]
+[[Category: Kuroki K]]
-[[Category: Maenaka, K]]
+[[Category: Maenaka K]]
-[[Category: Ose, T]]
+[[Category: Ose T]]
-[[Category: Rasubala, L]]
+[[Category: Rasubala L]]
-[[Category: Soler, N]]
+[[Category: Soler N]]
-[[Category: Yoshizawa, S]]
+[[Category: Yoshizawa S]]
-[[Category: Gtp-binding]]
-[[Category: Nucleotide- binding]]
-[[Category: Protein biosynthesis]]
-[[Category: Selenocysteine-specific elongation factor]]
-[[Category: Translation]]

2uwm

From Proteopedia

Current revision

C-TERMINAL DOMAIN(WH2-WH4) OF ELONGATION FACTOR SELB IN COMPLEX WITH SECIS RNA

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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